Invitrogen™ Novex™ AP Chemiluminescent Substrate (CDP-Star™), 100 mL

The Invitrogen™ Novex™ AP Chemiluminescent Substrate is a ready-to-use, non-radioactive detection reagent based on CDP-Star™, designed specifically for highly sensitive detection of alkaline phosphatase (AP)-conjugated antibodies in western blotting and other blot-based immunoassays such as dot blotting, Southern blotting, and Northern blotting.

This substrate enables chemiluminescence-based detection, offering exceptional sensitivity that surpasses traditional chromogenic methods. When AP-labeled antibodies are used, the CDP-Star substrate reacts enzymatically to emit light, which can be captured using X-ray film or CCD-based imaging systems. With detection sensitivity reaching low picogram levels, this system provides researchers with high signal intensity and low background for reliable and reproducible results.

Key Features

• High Sensitivity: Detects proteins at picogram levels, offering superior performance to chromogenic substrates.
• Non-Radioactive: Safe and convenient, eliminating the need for radioactive materials.
• Ready-to-Use Format: Preformulated liquid for direct application without additional preparation steps.
• Broad Application Compatibility: Suitable for Western, Southern, and Northern blotting workflows.
• Versatile Detection Options: Compatible with both X-ray film and digital imaging platforms.

Applications

• Western blot detection of AP-labeled primary or secondary antibodies.
• Dot blotting of nucleic acids or proteins.
• Southern blot hybridization signal detection.
• Northern blotting for RNA analysis.

Product Specifications

The Novex™ AP Chemiluminescent Substrate (CDP-Star™) from Invitrogen™ is a reliable, high-performance solution for researchers seeking sensitive and specific detection of AP-labeled targets in blotting assays. Its ready-to-use format, broad compatibility, and excellent detection range make it ideal for a wide range of molecular biology applications.