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Invitrogen™ CyQUANT™ MTT Cell Viability Assay, for 1000 Assays
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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The CyQUANT™ MTT Cell Viability Assay is a comprehensive kit designed to evaluate mammalian cell viability through a reliable and well-established colorimetric method. It utilizes the MTT reagent, which is reduced by viable cells to produce a pigmented formazan product. This product can be solubilized and quantified using a standard microplate reader, providing a straightforward and cost-effective way to assess cell viability.
Key Features
- Complete Kit:
- Includes all necessary reagents for approximately 1,000 assays in a 96-well plate format.
- Optimized for ease of use and reproducibility.
- Reliable MTT Reagent:
- Utilizes MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), a proven indicator of cellular redox potential.
- Converts to an insoluble formazan product in viable cells.
- Colorimetric Detection:
- Measures the optical density of the formazan product at 570 nm.
- Compatible with standard microplate absorbance readers.
- Applications:
- Cell viability assessment.
- Genotoxicity studies.
- Anti-cancer drug screening.
- General cell health evaluation.
- Ease of Use:
- Simple protocol: Add MTT reagent, incubate, solubilize, and read absorbance.
- Results in a straightforward readout for cell viability analysis.
Applications
- Cell Health Studies:
- Evaluate the effects of compounds on cell viability.
- Drug Screening:
- Assess cytotoxicity of anti-cancer or other therapeutic agents.
- Toxicology Studies:
- Measure genotoxicity in various experimental setups.
- Basic Research:
- Analyze the general health of mammalian cell cultures.
Specifications
| Feature | Details |
|---|---|
| Detection Method | Colorimetric |
| Format | 96-well microplate |
| Quantity | 1,000 assays |
| Readout | Optical density at 570 nm |
| Shipping Condition | Room temperature |
| Storage Conditions | Store at 2–8°C, protect from light |
| Compatible Equipment | Microplate reader |
| Dye Type | Other Labels or Dyes |
| Product Line | CyQUANT™ |
| Unit Size | 1,000 assays |
Advantages
- High Reliability:
- Uses the widely adopted MTT assay methodology for consistent results.
- Cost-Effective:
- Kit supports high-throughput workflows with 1,000 assay capacity.
- Compatibility:
- Compatible with standard laboratory microplate readers for convenience.
- Reproducibility:
- Optimized reagents ensure consistent and accurate results across assays.
Workflow
- Preparation:
- Prepare the MTT reagent according to the provided protocol.
- Incubation:
- Add the reagent to cells and incubate to allow reduction by viable cells.
- Formazan Solubilization:
- Add the included SDS reagent to solubilize the insoluble formazan product.
- Quantification:
- Measure the optical density at 570 nm using a microplate reader.
Why Choose the CyQUANT MTT Assay?
- Comprehensive kit for streamlined workflows.
- Trusted MTT-based method for precise cell viability evaluation.
- High throughput and cost efficiency, ideal for research labs.
- Reliable results for drug screening, toxicology, and cell health studies.
The Invitrogen™ CyQUANT™ MTT Cell Viability Assay is an essential tool for researchers needing reliable, reproducible, and efficient cell viability testing.