Thermo Scientific™ Pierce™ Fast Blocking Buffer

Rapid, Single-Protein Blocking Reagent for Western Blotting – Effective in Just 5 Minutes

Thermo Scientific™ Pierce™ Fast Blocking Buffer is a ready-to-use, single-protein-based blocking solution that significantly reduces western blotting workflow time. This buffer blocks PVDF and nitrocellulose membranes in just five minutes, helping minimize nonspecific binding while preserving strong, specific signal detection.

With performance comparable to traditional blocking buffers, Pierce Fast Blocking Buffer is compatible with biotin-avidin systems and common antibody formats. It is designed for seamless use with Pierce Fast Wash Buffer, which reduces total wash time to 20 minutes, making this system ideal for time-sensitive and high-throughput western blot protocols.

Key Features & Benefits

• Ultra-Fast Blocking – Achieves complete membrane blocking in just 5 minutes
• Low Background, High Signal Clarity – Minimizes nonspecific binding for crisp blot images
• Compatible with Nitrocellulose & PVDF – Versatile use across standard blotting membranes
• Biotin-Avidin System Compatible – Does not interfere with avidin-biotin-based detection
• Ready-to-Use Format – Supplied at 1X in TBST for immediate application
• Pair with Pierce Fast Wash Buffer – Reduces washing time from 90 minutes to just 20 minutes

Applications

• Traditional and high-throughput western blotting workflows
• Blocking PVDF or nitrocellulose membranes
• Use with HRP-, AP-, or fluorophore-conjugated antibodies
• Compatible with colorimetric, chemiluminescent, and fluorescent detection methods

Technical Specifications

Thermo Scientific™ Pierce™ Fast Blocking Buffer offers unmatched speed and consistency for western blot membrane blocking. With a quick 5-minute block time and compatibility with both nitrocellulose and PVDF membranes, it is a go-to reagent for researchers who need reliable performance and time efficiency in their immunoblotting workflows.