Superoxide Dismutase (Sod) - Elisa Kit, 96 Tests In 96 Well Plate

The Human Superoxide Dismutase (SOD) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of SOD levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into oxidative stress, antioxidant mechanisms, and related physiological or pathological conditions.

Purpose

• Quantification of SOD Levels:
  • Detect and measure SOD concentrations to study antioxidant defense mechanisms and oxidative stress.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the accurate and reliable quantification of SOD. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to SOD.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where SOD in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to SOD is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing SOD.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • SOD concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of SOD with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of SOD concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• SOD standards for standard curve generation.
• HRP-conjugated SOD detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Oxidative Stress Research:
  • Study SOD as a key enzyme in antioxidant defense systems.
• Antioxidant Mechanism Studies:
  • Investigate the role of SOD in mitigating reactive oxygen species (ROS).
• Clinical Diagnostics Research:
  • Assess SOD levels in conditions associated with oxidative stress, such as cardiovascular diseases, neurodegenerative disorders, and diabetes.
• Therapeutic Development:
  • Monitor SOD as a potential biomarker for therapeutic interventions targeting oxidative damage.
• Inflammation Studies:
  • Explore SOD levels in inflammatory responses and tissue injury.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of SOD.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated SOD antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate SOD concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Superoxide Dismutase (SOD) ELISA Kit is an essential tool for researchers studying oxidative stress, antioxidant defense, and related physiological and pathological conditions. Its precision, user-friendly design, and broad compatibility make it indispensable for a variety of clinical and experimental applications.