Sigma-Aldrich Human Serum, from human male AB plasma, USA origin, sterile-filtered

Sigma-Aldrich™ Human Serum (from male AB plasma, USA origin) is a sterile-filtered, high-purity serum supplement optimized for mammalian and stem cell culture. Derived from pooled AB male plasma, this product offers minimal immunoglobulin interference and supports a wide range of advanced cell culture applications, including tissue engineering, cell therapy, and transplantation modeling.

Human serum from AB donors is considered “universal” due to its lack of anti-A and anti-B antibodies, making it broadly compatible with human and hybrid cell lines. This particular product is obtained through natural clotting (off-the-clot), preserving an enriched mixture of growth factors and bioactive molecules critical for promoting optimal cellular proliferation, differentiation, and viability.

Key Features

• Human AB Plasma Origin: Minimizes antibody-related interactions; ideal for sensitive or immunologically relevant cell lines.
• Sterile-Filtered: Triple filtration ensures sterility for aseptic laboratory conditions.
• Endotoxin Tested: Strict quality control guarantees ≤10 EU/mL endotoxin—safe for stem cell and immune cell culture.
• Rich in Growth Factors: Naturally clotted serum retains a diverse array of growth-promoting proteins.
• Universal Serum: Ideal for multiple human cell types due to absence of A/B antigens.

Applications

• Stem Cell Culture:
  • Expansion of mesenchymal stem cells (MSCs) from adipose tissue or bone marrow.
  • Limbal epithelial stem cell graft development and transplantation.
• Immunotherapy Research:
  • Expansion of NK cells from peripheral blood in hematopoietic stem cell expansion media.
• Islet Cell Technology:
  • Optimization of human islet pre-transplant culture protocols.
• Analytical/Assay Use:
  • Component of PBST buffer in ELISA.
  • Mock biological matrix for testing ELISA formats (e.g., paper-based systems).
  • Diluent for standard curves in mAb quantification (e.g., NISTmAb assays).
  • Model system for studying serum behavior in multiple myeloma samples.

Specifications

Packaging & Safety Notes

• Packaging May Vary: Due to global supply limitations, bottle types and shrink wrap presence may differ between batches. These changes do not affect sterility or integrity.
• Cloudiness is Normal: Due to the lipid content in pooled human serum, visual cloudiness is expected and does not affect performance.
• Biosafety: Donors are ELISA-tested and found non-reactive for HIV, Hepatitis B & C. Nevertheless, the product should be handled as potentially infectious.

Sigma-Aldrich™ Human Serum from male AB plasma is a robust, high-quality supplement suitable for a wide range of human cell culture systems—especially where immunological neutrality and high growth factor content are essential. Its versatility in stem cell, immunology, and biochemical assay workflows makes it a key reagent in advanced biomedical research and therapeutic development.