Rifampicin Powder, Cell culture tested, 99%

Rifampicin, also known as Rifampin, is a semisynthetic antibiotic derived from rifamycin. It is particularly effective against gram-positive bacteria and shows moderate activity against gram-negative bacteria. This compound is cell culture tested, making it suitable for laboratory and pharmaceutical research applications. Rifampicin is widely used in the treatment of bacterial infections, including tuberculosis and leprosy, due to its ability to inhibit bacterial RNA synthesis.

Chemical and Physical Properties:

• Synonyms:
  • Rifampin
  • 3-(4-Methylpiperazinyliminomethyl)rifamycin SV
  • Rifamycin AMP
• Chemical Formula: C₄₃H₅₈N₄O₁₂
• Molecular Weight: 822.95 g/mol
• CAS Number: 13292-46-1
• MDL Number: MFCD00151389
• EINECS: 236-312-0

Physical Properties:

• Appearance: Bright red to orange crystalline powder
• Melting Point: 183–194°C
• Storage Temperature: Freezer (-20°C)
• Purity: Approximately 99%

Applications:

1. Pharmaceutical Research:
   • Tuberculosis Treatment: Rifampicin is a cornerstone drug for treating Mycobacterium tuberculosis infections. It is often used in combination with other antibiotics to prevent drug resistance.
   • Leprosy: Used in multidrug therapy for leprosy caused by Mycobacterium leprae.
   • Prophylaxis: Preventive treatment for individuals exposed to bacterial meningitis caused by Neisseria meningitidis.
2. Microbiological Research:
   • Antimicrobial Studies: Used to study its mechanism of inhibiting bacterial RNA polymerase, a critical enzyme in bacterial transcription.
   • Resistance Studies: Investigation into the development of rifampicin-resistant strains of bacteria.
3. Biochemical Research:
   • RNA Synthesis Studies: Rifampicin's role as an inhibitor of bacterial RNA synthesis makes it an important tool in studying transcriptional regulation.
   • Protein Expression Studies: Used in recombinant DNA technology to manage bacterial growth and protein production.
4. Cell Culture Applications:
   • Evaluated for use in cell culture systems to inhibit specific bacterial contaminants without adversely affecting eukaryotic cells.

Specifications:

• Pharmacological Action: Inhibits bacterial RNA polymerase, preventing transcription and leading to bacterial cell death.
• Activity Spectrum: Highly active against gram-positive bacteria and less effective against gram-negative bacteria.

Packaging Information:

• Pack Sizes and Catalog Numbers:
  • 1 g: Catalog No. APOSBIR0146-1G
  • 5 g: Catalog No. APOSBIR0146-5G
  • 25 g: Catalog No. APOSBIR0146-25G
• Packaging Type: Secure glass bottle

Storage and Handling:

• Storage:
  • Store at freezer temperatures (-20°C) to maintain stability. Protect from light and moisture.
• Handling Precautions:
  • Use appropriate personal protective equipment (PPE) such as gloves, safety goggles, and lab coats.
  • Avoid inhalation, ingestion, and direct contact with skin or eyes.

Key Features:

• Cell culture tested, ensuring reliability for laboratory research.
• High purity (approximately 99%) for consistent and reproducible results.
• Effective against gram-positive bacteria, including Mycobacterium species.
• Stable when stored at freezer temperatures, allowing for long-term use in research settings.

Apollo Scientific Rifampicin is a versatile antibiotic with critical applications in microbiology, pharmaceutical research, and cell culture. Its high purity and reliable activity make it an invaluable tool for studying bacterial transcription, resistance mechanisms, and disease treatment strategies. Whether for academic research or industrial applications, this compound provides exceptional performance and reproducibility.