Thermo Scientific™ Invitrogen™ Qubit™ Flex Fluorometer

The Invitrogen™ Qubit™ Flex Fluorometer is a high-performance benchtop fluorescence quantification instrument designed for precise and sensitive measurement of DNA, RNA, microRNA, protein, and endotoxin. Engineered for higher throughput and improved reproducibility, the Qubit Flex Fluorometer enables simultaneous quantification of up to eight samples, significantly reducing assay time and variability compared to single-sample fluorometers.

Using highly selective fluorescence-based detection with Qubit assay kits, the Qubit Flex system provides superior accuracy compared to traditional UV absorbance methods. Its intuitive touchscreen interface, built-in calculators, and flexible data export options make it an essential tool for genomics laboratories, sequencing facilities, and molecular biology research environments.

Key Features

High-Throughput Quantification

• Measures up to 8 samples simultaneously
• Up to 50% faster than single-sample fluorometers
• Reduces assay variability and improves reproducibility

Exceptional Sensitivity and Accuracy

• Fluorescence-based detection ensures highly accurate quantification
• Selective dyes detect only target molecules (DNA, RNA, protein, endotoxin)
• Eliminates interference from contaminants such as salts, free nucleotides, and proteins

Broad Quantification Capability

Supports quantification of:

• Double-stranded DNA (dsDNA)
• RNA
• microRNA
• Protein
• Endotoxin

Flexible Sample Requirements

• Requires only 1–20 μL sample volume
• Ideal for low-concentration and precious samples
• Compatible with a wide range of molecular biology workflows

Advanced User Interface

• Large 8-inch color touchscreen
• Intuitive and user-friendly interface
• Easy assay selection and workflow navigation

Built-in Calculators for Workflow Optimization

• Reagent preparation calculator
• Sample concentration calculator
• Molarity calculator for NGS workflows

Easy Data Export and Connectivity

• Export data via:
  • Wi-Fi dongle
  • USB drive
  • Network connection
• Supports CSV and PDF file formats

Instrument Verification and Compliance Support

• Pre-loaded performance verification program
• Optional 21 CFR Part 11 compliance software for regulated laboratories
• Ideal for quality-controlled environments

Applications

The Qubit Flex Fluorometer is ideal for a wide range of molecular biology and genomics applications, including:

• DNA quantification for next-generation sequencing (NGS)
• RNA quantification workflows
• microRNA analysis
• Protein quantification assays
• Endotoxin measurement
• Illumina sequencing library preparation workflows
• PCR and qPCR workflows
• Molecular cloning and genomics research

Advantages Over UV Spectrophotometry

Unlike UV absorbance instruments such as NanoDrop, the Qubit Flex Fluorometer provides:

• Higher sensitivity for low concentration samples
• Greater specificity for target molecules
• Reduced interference from contaminants
• More reliable quantification for sequencing workflows

This ensures improved accuracy and reduces the risk of sequencing failure due to incorrect input concentrations.

Technical Specifications

Kit Contents

• Qubit™ Flex Fluorometer
• Wi-Fi Dongle
• Power Cord
• SmartStart Digital Educational Experience Software

Advantages for Genomics and Sequencing Laboratories

• High-throughput DNA and RNA quantification
• Essential for Illumina NGS library preparation workflows
• Highly accurate fluorescence-based measurement
• Reduces variability and improves reproducibility
• Fast and efficient quantification workflow
• Ideal for genomics, biotechnology, and research laboratories

The Invitrogen™ Qubit™ Flex Fluorometer is a powerful, accurate, and high-throughput quantification instrument designed to meet the needs of modern genomics and molecular biology laboratories. Its ability to simultaneously measure multiple samples with exceptional sensitivity and accuracy makes it an essential tool for next-generation sequencing workflows, DNA and RNA analysis, and high-precision molecular quantification.