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Specifications:
| Application | Microbiology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Solid |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
Highly Nutritive, Non-Selective Culture Medium for the Isolation of Fastidious Organisms
Liofilchem® Chocolate Agar is a ready-to-use, enriched non-selective medium designed for the isolation and cultivation of fastidious microorganisms—particularly Neisseria spp. and Haemophilus spp.—from clinical specimens and other sanitary-relevant materials. The medium incorporates defibrinated horse blood and Vitalex Growth Supplement to provide essential growth factors such as hemin (X factor) and nicotinamide adenine dinucleotide (NAD or V factor), which are critical for the recovery of demanding bacterial species.
Recommended for clinical microbiology laboratories, this medium supports the growth of organisms requiring complex nutritional requirements and is frequently used in diagnostic testing, research, and public health investigations.
Key Features & Benefits
-
Highly Enriched Formula
- Contains enzymatic digests, corn starch, phosphates, and horse blood
- Supplemented with Vitalex Growth Supplement for enhanced recovery of Haemophilus and Neisseria
-
Supports Growth of Fastidious Bacteria
- Ideal for isolating Neisseria gonorrhoeae, Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae
-
Non-Selective & Nutrient-Rich
- Promotes general growth without inhibiting normal flora
- Allows for observation of colony morphology without the use of selective inhibitors
-
CO₂ Incubation Compatible
- Optimized for use in 5–10% CO₂ enriched aerobic atmospheres
-
Multiple Formats Available
- Plates, slant tubes, and bottles for flexibility in sample processing and culture maintenance
-
Ready-to-Use Convenience
- Reduces preparation time and risk of contamination in routine microbiology workflows
Applications
- Isolation of Neisseria and Haemophilus from genital, throat, and nasopharyngeal swabs
- Cultivation of Streptococcus pneumoniae and other fastidious respiratory pathogens
- Bacterial recovery from CSF, blood, sputum, and other clinical materials
- General-purpose growth of nutritionally demanding bacteria for identification and susceptibility testing
Typical Composition (per Liter)
| Component | Quantity |
|---|---|
| Enzymatic Digest of Casein | 7.5 g |
| Enzymatic Digest of Animal Tissue | 7.5 g |
| Corn Starch | 1.0 g |
| Dipotassium Phosphate | 4.0 g |
| Monopotassium Phosphate | 1.0 g |
| Sodium Chloride | 5.0 g |
| Agar | 17.0 g |
| Defibrinated Horse Blood | 50.0 mL |
| Vitalex Growth Supplement | 10.0 mL |
| Final pH | 7.2 ± 0.2 at 25°C |
Microbiological Quality Control (QC)
| Organism (ATCC®) | Expected Growth |
|---|---|
| Haemophilus influenzae ATCC® 10211 | Good |
| Neisseria gonorrhoeae ATCC® 43069 | Good |
| Neisseria meningitidis ATCC® 13090 | Good |
| Streptococcus pneumoniae ATCC® 6305 | Good |
| Streptococcus agalactiae ATCC® 13813 | Good |
Inoculum: 50–100 CFU
Incubation: 35 ± 2°C for 18–48 h in CO₂-enriched atmosphere
Product Formats & Ordering Information
| Format | Content | Ref. Code |
|---|---|---|
| 90 mm Ready-to-Use Plates | 20 plates | 10023 |
| 90 mm Ready-to-Use Plates | 100 plates | 10023* |
| Slant Tubes (Beaked, 9 mL) | 10 tubes | 30099 |
| Slant Tubes (Beaked, 9 mL) | 20 tubes | 31099 |
| Bottles (100 mL) | 6 bottles | 442220 |
| Bottles (500 mL) | 6 bottles | 470120 |
Preparation & Use Instructions
- Plates: Use directly after equilibration to room temperature.
- Slant Tubes/Bottles: Melt at 100°C (loosely capped), mix gently to ensure homogeneity, cool to 45–50°C, and pour into sterile Petri dishes under aseptic conditions.
- Inoculation: Streak specimens onto agar surface and incubate at 35 ± 2°C for 18–48 h in CO₂ atmosphere.
- Interpretation: Observe for bacterial growth; perform appropriate biochemical or immunological identification tests.
Storage & Shelf Life
| Storage Temperature | 2–8°C (protected from light)
| Shelf Life |
- Bottles/Tubes: 12 months
-
Ready-to-Use Plates: 5 months
| Transport Stability | Stable at 18–25°C for 4 days or 35–39°C for 48 hours
Liofilchem® Chocolate Agar provides a highly enriched, non-selective environment essential for the reliable recovery of fastidious organisms from clinical specimens. With flexible formats, stable shelf life, and excellent performance in CO₂-enriched incubation, this medium is a trusted choice for diagnostic, research, and reference laboratories handling critical microbiological investigations.
- Pack Size: 100/pk 20/Box