Liofilchem Novobiocin NO 30 µg

Liofilchem™ Novobiocin NO antimicrobial discs are precision tools designed for antimicrobial susceptibility testing (AST) in microbiology laboratories. These discs are available in 5 µg and 30 µg concentrations, ensuring versatility for both routine diagnostics and specialized applications. They are particularly effective in differentiating Staphylococcus saprophyticus from other Staphylococcus species, as well as assessing Novobiocin susceptibility in various bacterial strains.

Key Features

1. Two Concentrations for Versatility:
   • 5 µg: Used in specific diagnostic or experimental settings.
   • 30 µg: Widely utilized for routine testing, including the differentiation of Staphylococcus saprophyticus (Novobiocin-resistant).
2. Accurate and Reliable:
   • Ideal for Kirby-Bauer disk diffusion method to determine bacterial susceptibility to Novobiocin.
3. Quality Assured:
   • Manufactured under stringent controls to deliver consistent, reproducible results.
4. Ready-to-Use:
   • Pre-measured and individually sealed for immediate application in clinical, research, or environmental microbiology.
5. Broad Applicability:
   • Effective against Gram-positive bacteria and for identifying Novobiocin-resistant strains.

Applications

• Clinical Diagnostics:
  • Routine susceptibility testing for Staphylococcus species.
  • Differentiation of Staphylococcus saprophyticus from other staphylococci.
• Veterinary Microbiology:
  • Assessing antibiotic resistance in animal pathogens.
• Environmental and Food Microbiology:
  • Screening for Novobiocin-resistant organisms in environmental and food samples.
• Research:
  • Studying antimicrobial resistance patterns in various bacterial species.

Storage and Stability

• Long-term Storage: Keep at -20°C to maintain potency and efficacy.
• Short-term Storage: Store at 2–8°C for immediate or short-term use.
• Ensure discs are kept in a sealed, dry environment to preserve integrity.

Instructions for Use

1. Prepare a bacterial suspension adjusted to the appropriate turbidity standard (e.g., 0.5 McFarland).
2. Spread the suspension evenly on a Mueller-Hinton agar plate.
3. Place the Novobiocin NO disc (5 µg or 30 µg) on the agar surface using sterile forceps or a disc dispenser.
4. Incubate the plate at 35–37°C for 16–18 hours under aerobic conditions.
5. Measure the diameter of the inhibition zone and interpret results according to CLSI or EUCAST guidelines.

Advantages

• Precision: Delivers consistent and accurate inhibition zone readings.
• Flexibility: Two concentrations available for diverse testing needs.
• Ease of Handling: Ready-to-use discs compatible with standard laboratory workflows.

Packaging

• Supplied in blister packs to ensure sterility and longevity.
• Each pack contains 50 discs, individually sealed for optimal storage and handling.

Why Choose Liofilchem™ Novobiocin NO Discs?

Liofilchem™ Novobiocin NO discs offer precision, reliability, and ease of use for antimicrobial susceptibility testing. With options for long-term (-20°C) and short-term (2–8°C) storage, these discs are a convenient and dependable choice for microbiologists in clinical, veterinary, and research laboratories.