Roche KAPA SYBR® FAST, suitable for qPCR, 2 ×, Universal

The KAPA SYBR® FAST qPCR Kit is a highly efficient, versatile, and reliable solution for quantitative PCR (qPCR) using SYBR Green I dye chemistry. Designed with an engineered enzyme resistant to SYBR Green I inhibition, this kit ensures accurate, reproducible, and rapid qPCR performance for a wide range of applications.

Key Features

1. High Efficiency and Accuracy:
   • Amplification efficiencies between 95-105% for improved quantitation and reproducibility.
   • Reliable performance across a wide range of GC content and amplicon lengths.
2. Speed:
   • Fast cycling protocols allow complete qPCR runs in 40 minutes.
   • 55% shorter run times compared to standard protocols.
3. Enhanced Detection:
   • Detect low-copy and challenging targets consistently.
   • High fluorescence detection across AT- and GC-rich sequences.
4. Robust Design:
   • Engineered DNA polymerase optimized for SYBR Green I dye.
   • Resistant to dye inhibition and suitable for stringent reaction conditions.
5. Versatile Compatibility:
   • Compatible with major qPCR platforms, including:
     • Agilent Mx series (Mx3000P, Mx3005P, Mx4000)
     • Applied Biosystems (5700, 7000, 7500, 7900HT, ViiA 7, QuantStudio™, StepOne™, StepOnePlus™)
   • Suitable for gene expression, ChIP analysis, NGS library quantification, and more.

Kit Contents

• Concentration: 2X Master Mix with proprietary buffer.
• Includes: dNTPs and hot-start DNA polymerase.

Applications

1. Gene Expression Analysis
2. ChIP Assays
3. Gene Knockdown Validation
4. Microarray Validation
5. Absolute Quantification of NGS Libraries
6. Low-Copy Target Detection

Performance Benefits

• Efficient Amplification: Maintains unbiased efficiency for complex templates with variable GC content.
• Sensitive Detection: Improved processivity delivers earlier and consistent Cq values.
• Durable Stability: Resistant to light, stable for up to 30 freeze-thaw cycles, and retains functionality for one week at 4°C in the dark.

Storage and Handling

• Temperature: Store at -20°C protected from light.
• Short-Term Storage: Stable at 4°C for one week, provided no contamination occurs.
• Light Sensitivity: Protect from prolonged exposure to direct light to maintain fluorescence integrity.

Preparation Notes

1. Thawing and Mixing: Ensure components are fully thawed and mixed before use.
2. Reaction Setup: Do not exceed 25 μL reaction volumes.
3. Cycling Parameters:
   • Initial Denaturation: 20 sec at 95°C (or 3 min for complex templates).
   • Annealing/Extension: 20 sec for annealing; 1 sec for extension at 72°C (3-step cycling).

Compatibility

• Input: Purified DNA.
• Platforms: Works with a wide range of qPCR systems, including QuantStudio™, StepOne™, and Applied Biosystems series.

Packaging Options

Legal and Quality Information

• For Research Use Only: Not for diagnostic purposes.
• Certifications: Free from detectable exo- and endonuclease activity; stringent functional quality control applied.

This kit offers a robust, efficient, and versatile solution for all qPCR workflows, ensuring accurate and reproducible results in various research applications.