Invitrogen™ D-Biotin (Unconjugated)

Invitrogen™ D-Biotin is a high-purity, unconjugated form of biotin (also known as vitamin B7 or vitamin H) available in both liquid and powder formats. D-Biotin is widely used in molecular biology, immunoassays, affinity chromatography, and biochemical research due to its exceptionally high binding affinity to streptavidin, avidin, and NeutrAvidin™ proteins.

In its biologically active D-isomer form, biotin plays a key role in various enzyme cofactor functions and in labeling and detection workflows when paired with avidin-family binding proteins. It is a foundational reagent in biotin-streptavidin affinity systems commonly used in ELISA, blotting, and flow cytometry.

Key Features & Benefits

• High-Affinity Binding
  D-biotin forms one of the strongest known non-covalent bonds with streptavidin, avidin, and NeutrAvidin proteins—ideal for stable, high-sensitivity detection and capture assays.
• Versatile Applications
  Applicable in both chemical conjugation workflows and cellular labeling experiments, depending on format and protocol.
• Two Convenient Formats
  • Liquid (50 mM) format for immediate use in assays without dissolution (Cat. No. B20656)
  • Powder format for custom formulation and long-term storage (Cat. No. B1595)
• Unconjugated for Flexible Labeling
  Enables researchers to modify or conjugate biotin to their molecule of interest using appropriate coupling chemistries (e.g., NHS ester, maleimide, click chemistry).
• Compatible with Biotin-Binding Systems
  Fully compatible with all biotin-binding proteins used in detection and purification systems (e.g., streptavidin magnetic beads, NeutrAvidin enzyme conjugates).

Applications

• ELISA & Immunoassays
  Use as a component in biotin-streptavidin signal amplification systems for target detection.
• Protein & Nucleic Acid Labeling
  Modify antibodies, enzymes, or oligonucleotides for immobilization or capture.
• Affinity Purification
  Create biotinylated ligands for capture on streptavidin-coated beads or plates.
• Cell Surface & Intracellular Labeling
  For selective detection or imaging of biomolecules in live or fixed cell systems.
• Blotting Techniques
  Biotinylated probes or antibodies for Western, Southern, and Northern blot detection protocols.

Specifications

Invitrogen™ D-Biotin provides a high-affinity, biologically relevant biotin reagent for molecular biology, biochemistry, and immunodetection applications. Available in both liquid and powder forms, it offers flexibility for immediate use or custom biotinylation strategies. When combined with streptavidin-based detection or capture systems, it ensures consistent, high-performance results across a range of assay platforms.