Invitrogen™ Ambion™ RNase Inhibitor, cloned, 40 U/μL

The Invitrogen™ Ambion™ RNase Inhibitor is a highly potent recombinant ribonuclease inhibitor (RI) that effectively neutralizes pancreatic RNase A-type enzymes. Produced in E. coli and based on the human placental gene, this 50 kDa protein ensures superior RNA protection in workflows involving reverse transcription, RNA transcription, in vitro translation, and more.

By forming a tight 1:1 noncompetitive complex with target RNases, Ambion™ RNase Inhibitor safeguards RNA integrity even in environments with significant endogenous RNase activity. It is rigorously tested and free of RNase, DNase, exonuclease, endonuclease, and protease activity.

Key Features

✅ High Potency Inhibition

• Inhibits RNase A-type enzymes (e.g., RNase A, B, C) with high affinity (1:1 binding ratio)
• Protects RNA across a wide range of applications

✅ Wide pH Range and Stability

• Active from pH 5.0 to 8.0 (optimal: pH 7.0–8.0)
• Requires 1 mM DTT for activity preservation
• Stable and functional in most RNA-based reactions

✅ Compatible with Key Molecular Biology Workflows

• Ideal for:
  • Reverse transcription (cDNA synthesis)
  • In vitro transcription
  • In vitro translation
  • RNA isolation and storage

✅ Highly Pure, DNase-Free

• Free of contaminating RNases, DNases, exonucleases, endonucleases, and proteases
• Extensively quality controlled

✅ Flexible Use Format

• Supplied at 40 U/μL
• Recommended working concentration: 1 U/μL in reaction mixtures

Specifications

Unit Definition

One unit is defined as the amount required to inhibit the activity of 5 ng of RNase A by 50% under defined conditions (100 mM Tris-acetate, pH 6.5; 1 mM EDTA; 1 mM cyclic 2',3'-CMP).

Use Cases

🧬 cDNA Synthesis / Reverse Transcription (RT-PCR)

• Prevents degradation of RNA templates before and during first-strand synthesis

🧪 In Vitro Transcription and Translation

• Ensures high-quality RNA during enzyme-based synthesis and protein translation

🔬 RNA Storage / Purification

• Provides protection during RNA extraction and long-term storage

📦 Sensitive RNA Applications

• Critical in workflows involving low-abundance transcripts or degraded RNA samples

Ordering Information

Store at –20°C in a non-frost-free freezer.