Thermo Scientific™ Invitrogen™ Platinum™ II Hot-Start PCR Master Mix (2X)

High-Performance Hot-Start PCR for Reliable and Fast DNA Amplification The Invitrogen™ Platinum™ II Hot-Start PCR Master Mix (2X) is a ready-to-use, high-efficiency PCR master mix designed for fast, accurate, and high-throughput PCR applications. It contains Platinum II Taq Hot-Start DNA Polymerase, Platinum II PCR buffer, and dNTPs, allowing for universal primer annealing and superior specificity. Available in colorless and green formats, this master mix is optimized for genomic, viral, and plasmid DNA amplification, offering high resistance to inhibitors and robust performance with GC-rich templates.

Key Features & Benefits:

✅ Universal Primer Annealing at 60°C

• Innovative buffer stabilizes primer-template duplexes, eliminating the need for individual optimization.
• Allows different PCR assays to be cycled together using a single protocol.

✅ Fast & Robust Polymerase Performance

• Engineered Platinum II Taq DNA Polymerase enables rapid extension, delivering results 2X faster than standard Taq polymerases.
• Resistant to common PCR inhibitors, ensuring high sensitivity and yield.

✅ Hot-Start Technology for Superior Specificity

• Antibody-mediated hot-start prevents non-specific amplification at room temperature.
• Enhances sensitivity, specificity, and consistency.

✅ Optional Platinum GC Enhancer for GC-Rich Targets

• Improves PCR efficiency and yield for GC-rich sequences.
• Ensures reliable amplification of complex templates.

✅ Direct Gel Loading (Green Format Only)

• The Platinum II Hot-Start Green PCR Master Mix includes tracking dyes for direct loading of PCR products onto gels, reducing pipetting errors.

✅ Compatible with High-Throughput PCR & RT-PCR

• Ideal for automated workflows, genotyping, and high-throughput PCR.
• Compatible with RT-PCR workflows for cDNA amplification.

Available Formats & Catalog Numbers:

Technical Specifications:

Use Cases & Applications

1. High-Specificity Hot-Start PCR for Genomic DNA & Pathogen Detection
   • Primary Use: Amplification of DNA from complex genomic, viral, and plasmid templates.
   • Practical Example: A clinical diagnostics lab uses Platinum II Hot-Start PCR Master Mix to detect viral DNA from patient samples, ensuring high specificity and reproducibility.
2. Amplification of GC-Rich Targets
   • Primary Use: Ensures high-yield PCR for GC-rich sequences, reducing secondary structure interference.
   • Practical Example: A cancer research lab amplifies high GC-content promoter regions using Platinum GC Enhancer, ensuring consistent amplification across multiple samples.
3. Direct-to-Gel Loading for High-Throughput PCR Analysis
   • Primary Use: The Platinum II Green Master Mix allows direct loading of PCR products on agarose gels.
   • Practical Example: A university teaching lab uses Platinum II Green Master Mix for student PCR experiments, enabling faster analysis and reduced sample preparation steps.
4. Genotyping & SNP Analysis
   • Primary Use: Used for genotyping assays and SNP detection in agricultural and medical research.
   • Practical Example: A biotechnology company developing genetic markers for crop improvement uses Platinum II Hot-Start Master Mix for genotyping assays, ensuring consistent and reliable results.
5. RT-PCR for cDNA Amplification
   • Primary Use: Amplifies cDNA from RNA templates for gene expression analysis.
   • Practical Example: A molecular biology lab studying gene expression in mammalian cells uses Platinum II Hot-Start PCR Master Mix for cDNA amplification before qPCR analysis.

Why Choose Invitrogen™ Platinum™ II Hot-Start PCR Master Mix?

🔥 Universal Primer Annealing (60°C) – Eliminates optimization steps, allowing multiple assays in a single protocol. ⚡ Fast & High-Yield PCR – 2X faster results, ensuring efficient amplification of all DNA templates. 🛡 Hot-Start Technology for Specificity – Reduces non-specific amplification, improving sensitivity and reproducibility. 💡 GC-Rich Amplification with Platinum GC Enhancer – Enables successful amplification of difficult templates. 🔬 Direct Gel Loading (Green Format) – Reduces sample processing time in electrophoresis workflows.

The Invitrogen™ Platinum™ II Hot-Start PCR Master Mix (2X) is a powerful and flexible PCR solution for genomic research, pathogen detection, genotyping, and RT-PCR applications. Whether you need high-throughput automation, direct gel loading, or GC-rich amplification, this master mix delivers fast, consistent, and highly specific results.

For assistance with product selection, bulk orders, or pricing details, please contact LabMart Limited. We’re here to support your research success!