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Gibco™ DMEM, high glucose, GlutaMAX™ Supplement
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Stable, Nutrient-Rich Basal Medium for Enhanced Mammalian Cell Culture Performance
Gibco™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose, with GlutaMAX™ Supplement is a widely used, nutrient-rich basal medium designed to support the growth and maintenance of a wide range of mammalian cells. This formulation includes 4.5 g/L glucose, GlutaMAX™ (a stabilized form of L-glutamine), and phenol red, while excluding sodium pyruvate and HEPES.
GlutaMAX™ Supplement enhances medium stability by reducing ammonia buildup and degradation commonly associated with standard L-glutamine. This promotes improved cell viability and consistent performance in long-term and high-density cultures.
Key Features & Benefits
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High Glucose (4.5 g/L)
Supports high-energy-demanding cells and fast-growing cell lines. -
With GlutaMAX™ Supplement
A stabilized L-glutamine analog that improves shelf life and reduces toxic ammonia accumulation during culture. -
Phenol Red Indicator
Allows visual pH monitoring of culture conditions. -
Free of Sodium Pyruvate and HEPES
Allows for customized supplementation depending on experimental needs. -
Sterile & Ready to Use
Supplied in a sterile-filtered, 1X liquid format—just add serum and use. -
Manufacturing Quality
Produced under cGMP-compliant, ISO 13485-certified facilities for high lot-to-lot consistency.
Applications
- Routine mammalian cell culture and maintenance
- Primary cell expansion and differentiation
- Transfection, protein expression, and viral production
- Drug screening and toxicity assays
- Stem cell and neuronal culture support (with optimized supplementation)
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | DMEM, High Glucose with GlutaMAX™ |
| Product Line | Gibco™, GlutaMAX™ |
| Catalog Numbers | 10566016 (500 mL), 10566032 (5 L), 61965026 (500 mL), 61965240 (5 L), 61965059 (10 × 500 mL) |
| Concentration | 1X |
| Form | Liquid |
| Sterility | Sterile-filtered |
| With Additives | High Glucose, GlutaMAX™, Phenol Red |
| Without Additives | No Sodium Pyruvate, No HEPES |
| Cell Lines Supported | HeLa, 293, Cos-7, PC-12 |
| Primary Cells Supported | Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle |
| Buffer System | Sodium bicarbonate (3.7 g/L); requires 5–10% CO₂ |
| Storage Conditions | 2–8°C; protect from light |
| Shelf Life | 12 months from date of manufacture |
| Shipping Conditions | Ambient |
| Classification | Animal Origin-Free |
| Regulatory Status | For Research Use or Further Manufacturing Only |
Usage Guidelines
- Serum Supplementation: Add 5–10% Fetal Bovine Serum (FBS) or other supplement as required.
- CO₂ Incubation: Use in 5–10% CO₂ environment for proper pH balance.
- Handling: Use aseptic techniques to prevent contamination.
- Storage: Keep at 2–8°C and protect from light. Do not freeze.
Gibco™ DMEM, High Glucose, with GlutaMAX™ Supplement is an advanced basal medium optimized for robust, reproducible mammalian cell culture. Offering enhanced L-glutamine stability, high energy support, and flexible formulation, it is a go-to solution for researchers demanding high performance across a variety of cell types and experimental applications.
- Pack Size: 500 mL 10 x 500 mL 5L