Thermo Scientific™ SduI (Bsp1286I) (10 U/μL), 500 UNITS

Thermo Scientific™ SduI (Bsp1286I) Restriction Enzyme

The Thermo Scientific™ SduI (Bsp1286I) is a high-quality restriction enzyme that recognizes the sequence GDGCH^C and cleaves DNA at the specified site. This enzyme is optimized for use in its unique 10X buffer and offers reliable performance in molecular biology workflows. It is also available in the FastDigest™ format for rapid DNA digestion.

Recognition Site and Cleavage Pattern

• Recognition Sequence:
  • 5' G D G C H ↓ C 3'
  • 3' C H C G D ↑ G 5'
• Cleavage Details:
  • Cleaves within the recognition sequence, producing specific DNA fragments.
  • GDGCH represents a degenerate sequence:
    • G = guanine
    • D = A, G, or T
    • H = A, C, or T
• Isoschizomers:
  • Enzymes with the same recognition site include Bsp1286I and MhlI.

Buffer and Reaction Conditions

1. Unique Buffer System:
   • Works optimally with its designated 10X SduI (SbfI) buffer.
   • Premixed with BSA for enhanced enzyme stability and to bind potential DNA contaminants.
2. Compatibility with Tango Buffer:
   • The universal Tango Buffer facilitates double digestions with other enzymes.
3. Optimal Reaction Temperature:
   • Functions best at 37°C, making it suitable for standard molecular biology workflows.
4. Heat Inactivation:
   • Sensitive to heat inactivation and can be inactivated by heating at a specified temperature after the reaction is complete.

Features

• Superior Quality:
  • Manufactured with stringent quality controls to ensure consistency and reliability.
• Color-Coded Buffer System:
  • Simplifies enzyme selection and setup with the Five Buffer System.
• Convenience for Double Digestions:
  • Includes the universal Tango buffer for dual enzyme reactions.
• Premixed BSA:
  • Stabilizes the enzyme and minimizes contaminants' effects.
• Wide Specificity:
  • Part of a diverse collection of restriction endonucleases catering to various applications.

Applications

The SduI enzyme is versatile and suitable for numerous molecular biology applications, including:

• Molecular Cloning:
  • Precision DNA cutting for cloning and recombinant DNA experiments.
• Restriction Site Mapping:
  • Identifying and characterizing restriction sites in DNA sequences.
• Genotyping:
  • Differentiating genetic variations based on restriction patterns.
• Southern Blotting:
  • Detecting specific DNA sequences through hybridization.
• RFLP (Restriction Fragment Length Polymorphism):
  • Analyzing genetic diversity or mutations.
• SNP (Single Nucleotide Polymorphism) Analysis:
  • Detecting and characterizing SNPs in genomic DNA.

Specifications

• Concentration:
  • 10 U/μL (500 units per package).
• Methylation Sensitivity:
  • Not sensitive to Dam, Dcm, or CpG methylation, ensuring consistent cutting in methylated DNA contexts.
• Buffer Compatibility:
  • Works with its unique buffer and is compatible with Tango for double digestions.
• Heat Inactivation:
  • Heat-sensitive; ensure proper deactivation after use.
• Reaction Temperature:
  • Optimal at 37°C.
• Enzyme Type:
  • Type II restriction enzyme (not Type IIS).

Contents and Storage

Each package includes:

• 500 units of SduI enzyme (10 U/μL concentration).
• 10X Buffer SduI (SbfI) for optimal enzyme activity.

Storage:

• Store at –20°C to maintain enzyme stability.

Notes and Considerations

1. FastDigest Availability:
   • SduI is also available as a FastDigest enzyme, offering faster digestion (5–15 minutes) and universal buffer compatibility.
2. Downstream Applications:
   • Enzyme's compatibility with cloning, genotyping, and Southern Blotting workflows makes it a versatile choice for researchers.
3. For Research Use Only:
   • This enzyme is intended for research purposes and not for diagnostic use.

The Thermo Scientific™ SduI (Bsp1286I) enzyme is a high-performance, reliable tool for DNA digestion, offering flexibility with its unique buffer and Tango Buffer system. Its precise cleavage, versatility in applications, and ease of use make it a valuable choice for molecular biology and genetic research workflows.