Thermo Scientific™ BL21(DE3) Competent Cells

Thermo Scientific™ BL21(DE3) Competent Cells are chemically competent E. coli cells that are optimized for the expression of heterologous genes, especially those requiring the T7 RNA polymerase system. These cells are engineered with the lambda DE3 prophage, which carries the T7 RNA polymerase gene under control of the lacUV5 promoter. This setup allows for the inducible expression of T7 RNA polymerase with IPTG, enabling high-level expression of recombinant proteins in E. coli.

BL21(DE3) cells are genetically modified to reduce protein degradation by lacking the lon protease and the outer membrane protease OmpT, which helps preserve the integrity of expressed recombinant proteins.

Key Features & Benefits:

1. High Transformation Efficiency:
   • >1x10⁷ cfu/μg of pUC19 DNA, ensuring excellent transformation results for recombinant DNA applications.
2. Optimal for Recombinant Protein Expression:
   • Specifically designed for the high-level expression of recombinant proteins using the T7 expression system, which is highly efficient and widely used in protein expression studies.
3. Protease Deficiency:
   • BL21(DE3) cells lack the lon protease and OmpT protease, reducing the degradation of expressed proteins, and providing more stable production of heterologous proteins.
4. Convenient Packaging:
   • Supplied in a convenient two-reaction vial packaging, making it easier to handle and use in experiments. This packaging also includes S.O.C. outgrowth medium for efficient recovery of transformed cells.
5. Genetic Markers:
   • The DE3 genotype ensures that the expression of the T7 RNA polymerase gene can be induced by IPTG, facilitating controlled expression of your gene of interest.

Applications:

• Recombinant Protein Expression: Ideal for high-level expression of recombinant proteins using the T7 expression system, widely used in molecular biology, biotechnology, and pharmaceutical research.
• Gene Cloning: Suitable for cloning experiments that require high-efficiency transformation and protein expression.
• Protein Purification: Frequently used for the production and purification of recombinant proteins for research, structural studies, and therapeutic development.

Genotype:

• F– ompT hsdSB (rB–, mB–) gal dcm (DE3)

Specifications:

Contents:

• 10 x 100 μL of BL21(DE3) Competent Cells
• 50 μL of pUC19 DNA (10 pg/μL)
• 5 mL of S.O.C. Medium

Storage:

• Store competent cells at -80°C for long-term storage.

Thermo Scientific™ BL21(DE3) Competent Cells are optimized for high-efficiency transformation and recombinant protein expression using the T7 RNA polymerase system. Their lack of key proteases ensures minimal degradation of your expressed proteins, making them ideal for applications in protein expression, gene cloning, and biotechnology research.