CellTiter-Glo® Luminescent Cell Viability Assay

The CellTiter-Glo® Luminescent Cell Viability Assay is a highly sensitive, homogeneous assay for determining cell viability based on the quantitation of ATP, a key indicator of metabolically active cells. Its streamlined "add-mix-measure" protocol makes it ideal for high-throughput screening (HTS), cytotoxicity testing, and cell proliferation studies.

Key Features:

1. ATP-Dependent Detection:
   • Measures ATP as an indicator of metabolically active and viable cells.
2. High Sensitivity:
   • Detects as few as 15 cells/well in a 384-well format with up to 5 logs linear range.
3. Homogeneous Protocol:
   • No cell washing, medium removal, or multiple pipetting steps required.
4. Extended Signal Stability:
   • Produces a "glow-type" luminescent signal with a half-life of >5 hours, providing flexibility for batch or continuous processing.
5. Automation Friendly:
   • Designed for use in multiwell formats, compatible with automated HTS workflows.
6. Cost-Efficient Scaling:
   • Available in multiple sizes for scalability from bench experiments to high-throughput screening.

Applications:

1. High-Throughput Screening (HTS):
   • Quickly evaluates cell viability and metabolic activity in large-scale drug discovery and screening programs.
2. Cytotoxicity Testing:
   • Measures the impact of compounds, RNAi, or gene editing on cell viability.
3. Cell Proliferation Studies:
   • Quantifies the growth rate of cell lines under different experimental conditions.
4. Antiviral Research:
   • Assesses the cytopathic effects of viral infections, including SARS-CoV and SARS-CoV-2.
5. Cancer Research:
   • Tracks cell viability during gene knockdown experiments targeting noncoding RNAs or other potential therapeutic targets.

Specifications:

Advantages:

1. Ease of Use:
   • Simple protocol minimizes errors and increases reproducibility.
2. Extended Signal Stability:
   • Eliminates the need for reagent injectors, allowing flexibility in assay timing.
3. Versatile Applications:
   • Applicable to various cell types and experimental conditions.
4. Automation Ready:
   • Compatible with robotic liquid handlers and luminescent plate readers.
5. Cost-Efficient:
   • Available in multiple sizes to support both small-scale experiments and high-throughput applications.

Kit Sizes and Capacity:

Storage and Handling:

• Storage Conditions: Store at -20°C to maintain reagent stability.
• Working Solution: Prepare fresh by adding CellTiter-Glo® reagent directly to cells; mix and measure luminescence within 10 minutes.

Use Cases:

1. Drug Discovery:
   • Screens small molecules for cytotoxic or therapeutic effects on cell lines.
2. Antiviral Studies:
   • Evaluates the effectiveness of antiviral compounds using cytopathic effect assays.
3. RNAi and CRISPR Experiments:
   • Monitors cell viability post-knockdown or gene editing.
4. Basic Research:
   • Tracks cell proliferation and metabolic activity under varying experimental conditions.

Ordering Information:

The CellTiter-Glo® Luminescent Cell Viability Assay is a trusted, high-sensitivity method for measuring ATP levels, providing reliable insights into cell health, viability, and proliferation. Its robust, easy-to-use format and compatibility with high-throughput workflows make it an essential tool for researchers in drug discovery, cancer biology, and virology.