Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit

The Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit provides a reliable and efficient system for first-strand cDNA synthesis, supporting a wide range of RNA templates, including low-expression transcripts and GC/AU-rich regions. The kit enables the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a standard 20 µL reaction format, and up to 10 µg of cDNA in a 100 µL scaled-up reaction.

Available with or without RNase Inhibitor, the kit ensures maximum template protection, high yields, and exceptional reproducibility across your reverse transcription workflows.

Key Features

✅ Robust Performance

• Converts 0.02–2 μg of total RNA to cDNA per 20 μL reaction
• Scalable to 100 μL reactions, producing up to 10 μg of cDNA

🧬 High-Quality First-Strand cDNA

• Delivers cDNA suitable for qPCR, RT-PCR, traditional PCR, microarrays, and cDNA archiving

🎯 Greater Accuracy & Dynamic Range

• Provides linear target amplification, crucial for quantitative applications
• 10-fold greater dynamic range than comparable kits
• Higher yields and precision at a lower cost

🔬 Tested for a Wide Range of Templates

• Validated across GC- and AU-rich RNA, and low-abundance transcripts
• Uses random primers for unbiased first-strand synthesis
• Ideal for total RNA, poly(A)+ mRNA, and in vitro transcribed RNA

🔐 RNase Protection (Kit w/ Inhibitor)

• RNase Inhibitor prevents RNA degradation and improves yields
• Recombinant, DNA endonuclease-free enzyme for higher specificity

Kit Contents & Storage

Storage Conditions: –15°C to –25°C

Shipping: Shipped on dry ice

Specifications

Ordering Information

For Research Use Only. Not for use in diagnostic procedures.