Unperforated Cellulose Acetate Membrane Papers, 57X140Mm, 50/Pack

Cellulose acetate membrane papers are widely used in laboratory applications requiring precise separation and analysis of biomolecules. The Dry Unperforated Cellulose Acetate Membrane Papers (57x140mm) are designed for high-resolution hemoglobin electrophoresis, providing a reliable and consistent medium for separating and identifying different hemoglobin variants.

Product Overview

These cellulose acetate membrane papers are unperforated, dry, and come in dimensions of 57x140mm, making them suitable for standard electrophoresis equipment. The product is supplied in a pack of 50 sheets, ensuring sufficient membranes for routine and high-throughput laboratory workflows.

Applications

1. Hemoglobin Electrophoresis:
   • Separation and identification of hemoglobin variants, including HbA, HbF, HbS, and HbC.
   • Essential for diagnosing hemoglobinopathies such as sickle cell anemia and thalassemias.
   • Suitable for screening and diagnostic use in clinical and research laboratories.
2. Protein Separation:
   • Analysis of proteins based on their charge-to-mass ratio under an electric field.
   • Used in both qualitative and semi-quantitative assays.
3. Molecular Testing:
   • Ideal for routine separation of small molecules and nucleic acids, although primarily optimized for hemoglobin.

Key Features

1. High Purity Material:
   • Made from cellulose acetate, ensuring compatibility with electrophoretic buffers and biological samples.
   • Minimizes background noise and enhances clarity of results.
2. Consistent Performance:
   • Uniform pore structure and thickness for reproducible separations.
   • Optimal resolution for distinguishing closely migrating hemoglobin variants.
3. Unperforated Design:
   • Ensures a smooth and uninterrupted surface, which is critical for consistent electrophoretic migration and clear band formation.
4. Dry Format:
   • Eliminates the need for pre-hydration, making the membranes ready to use after buffer application.
5. Ideal Size:
   • The 57x140mm dimensions are tailored to fit most electrophoresis chambers used in hemoglobin testing.
6. Pack of 50:
   • Cost-effective for high-throughput settings or frequent testing requirements.

Technical Specifications

Advantages for Hemoglobin Electrophoresis

1. High Resolution:
   • Effectively separates hemoglobin variants with minimal overlap.
2. Reproducibility:
   • Provides consistent results across multiple tests, critical for diagnostic accuracy.
3. Ease of Use:
   • Requires minimal preparation and integrates seamlessly into standard electrophoretic protocols.
4. Cost Efficiency:
   • Bulk pack of 50 membranes reduces cost per test and minimizes wastage.

Usage Protocol for Hemoglobin Electrophoresis

1. Membrane Preparation:
   • Soak the cellulose acetate membrane in the appropriate electrophoresis buffer for a few minutes before use to ensure even hydration.
2. Sample Application:
   • Apply hemolysates or patient samples onto the membrane using an applicator.
3. Electrophoresis:
   • Place the membrane in the electrophoresis chamber filled with the corresponding buffer.
   • Run the electrophoresis at the recommended voltage and duration.
4. Staining and Visualization:
   • After electrophoresis, stain the membrane to visualize hemoglobin bands.
   • Use a densitometer or imaging system for band quantification if required.

Storage and Handling

• Store in a cool, dry place away from direct sunlight and moisture to maintain product integrity.
• Handle with clean gloves to prevent contamination.

Who Should Use This Product?

• Clinical Laboratories: For routine hemoglobinopathy screening and diagnostic workflows.
• Research Institutions: For studying hemoglobin variants or other protein separation applications.
• Biotechnology and Pharmaceutical Labs: For quality control and molecular testing purposes.

The Dry Unperforated Cellulose Acetate Membrane Papers (57x140mm) offer a high-performance, reliable solution for hemoglobin electrophoresis. Their consistent quality, ease of use, and compatibility with standard laboratory protocols make them an essential tool for laboratories focused on protein separation and diagnostic testing.