Liofilchem® Miconazole, Antimicrobial Susceptibility Discs, MCL 10ug

Liofilchem® Metronidazole MTZ Discs (5 µg) are high-quality in vitro diagnostic tools designed for antimicrobial susceptibility testing (AST) using the Kirby-Bauer disk diffusion method. Each disc is impregnated with 5 micrograms of Metronidazole, a nitroimidazole-class antibiotic primarily active against anaerobic bacteria and protozoa. These discs are manufactured under strict ISO 9001 and ISO 13485 quality standards to ensure consistent performance, sterility, and accuracy for routine clinical microbiology and antimicrobial resistance monitoring.

Key Features

• Standardized antibiotic content: Each disc contains 5 µg of Metronidazole for accurate and reproducible AST results.
• Anaerobe-specific activity: Effective against Bacteroides fragilis, Clostridium spp., Fusobacterium spp., Prevotella spp., and Gardnerella vaginalis.
• Validated for anaerobic susceptibility testing: Suitable for use with Brucella Blood Agar or Wilkins-Chalgren Anaerobic Agar under anaerobic conditions.
• Sterile and shelf-stable: Packaged in sealed canisters or cartridges with desiccant to maintain potency.
• CLSI- and EUCAST-compatible: Disc content and method align with international AST standards.

Applications and Use Cases

1. Clinical Microbiology

Used in hospital and diagnostic labs to:

• Determine susceptibility of obligate anaerobes from:
  • Intra-abdominal infections
  • Gynecological infections (e.g., bacterial vaginosis)
  • Oral, dental, and periodontal abscesses
  • Clostridial infections (e.g., Clostridium difficile)
• Support effective treatment with metronidazole or related nitroimidazoles.

2. Resistance Surveillance and Stewardship

• Monitor regional and institutional resistance patterns in anaerobic pathogens.
• Detect reduced susceptibility in Bacteroides and Clostridium isolates.

3. Research and Development

• Assess metronidazole activity in experimental treatment protocols.
• Study resistance mechanisms (e.g., nim genes, efflux pumps) in anaerobic species.
• Use in quality control and comparative activity testing of antianaerobic agents.

Use Instructions

1. Prepare anaerobic media such as Brucella Blood Agar or Wilkins-Chalgren Anaerobic Agar with vitamin K and hemin.
2. Standardize the inoculum to 0.5 McFarland using anaerobic suspensions.
3. Inoculate the agar surface uniformly using a sterile swab.
4. Apply the Metronidazole disc (5 µg) using sterile forceps or an automatic disc dispenser.
5. Incubate at 35–37 °C for 24–48 hours in an anaerobic chamber or jar.
6. Measure the diameter of the inhibition zone in millimeters.
7. Interpret results using CLSI M100 or EUCAST anaerobe-specific breakpoints.

Storage and Shelf Life

• Storage Conditions: 2–8 °C (refrigerated), in sealed packaging with desiccant.
• Shelf Life: 24–36 months from the date of manufacture when properly stored.

Liofilchem® Metronidazole MTZ Discs (5 µg) provide an accurate, reliable method for evaluating susceptibility to one of the most widely used antibiotics for anaerobic infections. These discs are essential in guiding effective therapy, monitoring resistance, and supporting laboratory surveillance. Manufactured to meet international standards, they are a trusted choice for clinical microbiology laboratories, infectious disease centers, and research institutions.