Liofilchem® Ciprofloxacin CIP, Antimicrobial Susceptibility Discs, 5 µg

The Liofilchem® Ciprofloxacin Antimicrobial Susceptibility Discs (5 µg) are specialized tools designed for the Kirby-Bauer Disk Diffusion Method, a standardized assay used to evaluate the susceptibility of bacterial pathogens to the antibiotic ciprofloxacin. These discs are essential in clinical microbiology and research laboratories to guide antimicrobial therapy and monitor bacterial resistance trends.

Key Features

1. Active Ingredient:
   • Contains 5 µg of ciprofloxacin, a broad-spectrum fluoroquinolone antibiotic effective against Gram-positive and Gram-negative bacteria.
2. High-Quality Standards:
   • Manufactured in compliance with CLSI (Clinical and Laboratory Standards Institute) and EUCAST (European Committee on Antimicrobial Susceptibility Testing) guidelines.
   • Ensures consistency and reproducibility in results.
3. Application:
   • Used in the Kirby-Bauer Disk Diffusion Test to measure zones of inhibition, determining bacterial susceptibility or resistance to ciprofloxacin.
4. Packaging:
   • Supplied in convenient cartridges or packs to maintain sterility and usability.
   • Suitable for routine use in clinical, diagnostic, and research laboratories.
5. Material:
   • Made of high-quality filter paper impregnated with a precise amount of ciprofloxacin to ensure accurate diffusion and reliable results.

Applications

1. Clinical Diagnostics:
   • Evaluates the effectiveness of ciprofloxacin against bacterial pathogens causing infections, such as:
     • Escherichia coli
     • Pseudomonas aeruginosa
     • Staphylococcus aureus
     • Enterobacteriaceae
     • Neisseria gonorrhoeae
2. Antibiotic Resistance Monitoring:
   • Detects ciprofloxacin resistance, a growing concern in Gram-negative bacteria and other pathogens.
3. Research:
   • Used in studies investigating bacterial resistance mechanisms and the efficacy of ciprofloxacin.
4. Infection Control:
   • Helps inform treatment strategies in hospital and community healthcare settings to prevent the spread of resistant strains.

Testing Protocol: Kirby-Bauer Disk Diffusion Method

1. Preparation:
   • Prepare a bacterial suspension adjusted to a 0.5 McFarland standard.
2. Inoculation:
   • Swab the suspension evenly onto a Mueller-Hinton agar plate.
3. Application:
   • Place the Liofilchem® Ciprofloxacin 5 µg disc onto the agar surface using sterile forceps or a disc dispenser.
4. Incubation:
   • Incubate the plate at 35 ± 2°C for 16–18 hours under aerobic conditions.
5. Interpretation:
   • Measure the zone of inhibition around the disc.
   • Compare with CLSI or EUCAST interpretive criteria to classify the organism as susceptible (S), intermediate (I), or resistant (R).

Regulatory Compliance

• ISO 9001 Certified: Ensures manufacturing consistency and quality.
• CLSI and EUCAST Standards: Fully compliant with global susceptibility testing guidelines.
• CE Marked: Approved for in vitro diagnostic use in Europe.

Clinical Importance

1. Broad-Spectrum Activity:
   • Ciprofloxacin is effective against many Gram-negative and some Gram-positive bacteria, including Enterobacteriaceae, Pseudomonas spp., and Staphylococcus spp..
2. Resistance Concerns:
   • Increasing resistance, particularly in E. coli and Klebsiella pneumoniae, necessitates routine testing to ensure ciprofloxacin efficacy.
3. Guiding Therapy:
   • Provides essential data to inform antibiotic selection and dosage for infections, including urinary tract infections, respiratory infections, and septicemia.

Comparison with Similar Products

The Liofilchem® Ciprofloxacin 5 µg Antimicrobial Susceptibility Discs are reliable, high-quality tools for assessing bacterial susceptibility to ciprofloxacin. They meet international standards for clinical diagnostics and are essential for guiding antimicrobial therapy, monitoring resistance trends, and supporting infection control measures. Their cost-effectiveness and compliance with CLSI and EUCAST guidelines make them a preferred choice for laboratories worldwide.