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Liofilchem® Middlebrook 7H10 (OADC) supplement 4 x 50 mL
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
Liofilchem® Middlebrook 7H10 (OADC) Supplement is a sterile, ready-to-use liquid enrichment supplement used for the preparation of Middlebrook 7H10 or 7H11 agar media for the cultivation of mycobacteria, including Mycobacterium tuberculosis. It contains a carefully balanced formulation of oleic acid, bovine albumin (fraction V), glucose, catalase, and sodium chloride, which support the nutritional and physiological needs of mycobacteria during in vitro culture.
The supplement is used in combination with Middlebrook 7H10 Agar Base (codes: 611022 or 621022) or Middlebrook 7H11 Agar Base (codes: 610213 or 620213) to enhance recovery and growth of M. tuberculosis and other mycobacteria.
Key Features and Benefits
- ✅ Growth-promoting formulation: Supports optimal growth and metabolic activity of mycobacteria.
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✅ Contains protective and energy-enhancing agents:
- Oleic acid – Long-chain fatty acid critical for cell wall synthesis and energy.
- Bovine albumin – Binds and neutralizes toxic compounds, enhancing mycobacterial recovery.
- Glucose – Readily metabolized carbon and energy source.
- Catalase – Destroys harmful peroxides.
- Sodium chloride – Maintains osmotic balance in the medium.
- ✅ Compatible with both Middlebrook 7H10 and 7H11 agar bases.
- ✅ Reliable performance: Quality-controlled using standard mycobacterial strains.
- ✅ Long shelf life: Stable when stored at 2–8 °C in sealed packaging.
Composition (per 50 mL vial)
| Component | Per Bottle (50 mL) | Equivalent per Liter of Medium |
|---|---|---|
| Oleic Acid | 0.03 mL | 0.06 mL |
| Bovine Albumin (V) | 2.5 g | 5.0 g |
| Glucose | 1.0 g | 2.0 g |
| Catalase | 0.0015 g | 0.003 g |
| Sodium Chloride | 0.425 g | 0.85 g |
| Distilled Water | to 50.0 mL | — |
Instructions for Use
- Autoclave and cool 450 mL of Middlebrook 7H10 or 7H11 Agar Base to 45–50 °C.
- Aseptically add the entire content (50 mL) of one bottle of Middlebrook 7H10 (OADC) Supplement.
- Mix thoroughly to ensure even distribution.
- Dispense into sterile containers or Petri dishes and allow to solidify under aseptic conditions.
Microbiological Quality Control
| Control Strain | ATCC | Expected Growth |
|---|---|---|
| Mycobacterium scrofulaceum | ATCC 19981 | Good |
| Mycobacterium intracellulare | ATCC 13950 | Good |
Incubation conditions: 36 ± 1 °C for 21 days in an atmosphere containing 10% CO₂.
Appearance
- Amber-colored liquid
- Clear or slightly opalescent
Storage and Stability
- Storage temperature: 2–8 °C
- Shelf life: Up to the expiry date printed on the label
- Do not use if signs of deterioration (e.g., precipitation, discoloration) are present
Precautions
- For in vitro diagnostic use only
- Use in a professional laboratory setting by trained personnel
- Follow appropriate biosafety procedures when handling mycobacteria
References
- Dubos & Middlebrook, Am. Rev. Tuberc. 1947
- Middlebrook & Cohn, Am. J. Pub. Health 1958
- Middlebrook et al., Acta Tubercul. Scand. 1960
Liofilchem® Middlebrook 7H10 (OADC) Supplement is an essential enrichment component for preparing Middlebrook media used in the isolation and cultivation of Mycobacterium tuberculosis and other mycobacteria. Its optimized formulation supports robust growth, enhances recovery during primary isolation, and ensures compatibility with standard mycobacterial culture protocols. Backed by ISO-certified manufacturing and microbiological quality control, this supplement is a trusted choice for clinical microbiology laboratories, TB reference centers, and research institutions focused on mycobacterial diagnostics and surveillance.