Liofilchem Lactose Broth

The Liofilchem® Lactose Broth is a reliable medium designed for the detection of Salmonella and coliforms in water, food, dairy, and pharmaceutical products. This dehydrated medium is ideal for pre-enrichment, ensuring accurate results for microbial testing in compliance with regulatory standards.

Key Features

1. Comprehensive Detection:
   • Suitable for identifying Salmonella and coliforms in diverse samples including water, dairy, food, and pharmaceuticals.
2. Robust Formulation:
   • Contains beef extract, peptone, and lactose to support microbial growth and fermentation detection.
3. Versatile Application:
   • Supports pre-enrichment and selective plating for microbial isolation.
4. Reliable Gas Production Indicator:
   • Lactose fermentation is identified through gas production, simplifying result interpretation.

Specifications

Formula

Physical Properties

Environmental and Storage

Packaging Options

Applications

1. Water Testing:
   • Detects coliforms and Salmonella in water samples for regulatory compliance.
2. Food Safety:
   • Ensures microbial safety in dairy and processed food products.
3. Pharmaceutical Monitoring:
   • Validates cleanliness and microbial limits in pharmaceutical manufacturing.
4. Dairy Industry:
   • Detects contamination in milk and related products.

Usage Guidelines

Preparation

1. Suspend 13 g of powder in 1 liter of distilled or deionized water.
2. Heat until completely dissolved.
3. Dispense into sterile containers with or without Durham tubes.
4. Autoclave at 121°C for 15 minutes.

Technique

1. Pre-enrichment:
   • Mix 25 g/mL of the sample with 225 mL of Lactose Broth and incubate at 35°C for 24 ± 2 hours.
2. Enrichment:
   • Transfer 1 mL of the pre-enrichment broth into a suitable enrichment broth and incubate.
3. Selective Plating:
   • Streak a loopful onto an appropriate agar plate.

Results Interpretation

• Gas production indicates lactose fermentation.
• Use selective plating and enrichment to isolate Salmonella and coliforms effectively.

Microbial Performance

Advantages

1. Ease of Use:
   • Simplified preparation and reliable results.
2. Regulatory Compliance:
   • Supports APHA and industry standards for microbial testing.
3. Versatility:
   • Suitable for a range of samples and applications.
4. High Shelf Life:
   • Stable for 4 years under recommended conditions.

Warnings and Precautions

1. Use only by trained personnel in a laboratory setting.
2. Store in a dry environment and avoid contamination.
3. Dispose of waste in accordance with local and national regulations.

References

1. APHA (1985). Standard Methods for the Examination of Water and Wastewater, 16th ed.
2. APHA (1972). Standard Methods for the Examination of Dairy Products, 13th ed.
3. Flowers, R.S., et al. (1992). Salmonella, p. 371-442.