The Liofilchem® Malt Extract Agar is a solid culture medium specifically designed for the isolation, cultivation, and enumeration of yeasts and molds. It provides an acidic environment that promotes the growth of fungi while restricting bacterial contamination.
Key Features
- Optimized for Fungal Growth:
- Supports a wide range of yeasts and molds with its nutrient-rich formula.
- Reliable Detection:
- Acidic pH restricts bacterial growth while encouraging fungal proliferation.
- Convenient Preparation:
- Dehydrated format allows for easy and consistent preparation.
- Broad Applications:
- Ideal for food, environmental, and pharmaceutical fungal testing.
Specifications
Formula
Component | Amount (g/L) |
---|
Maltose | 12.75 |
Dextrin | 2.75 |
Peptone | 0.78 |
Agar | 15.0 |
Final pH (at 25°C) | 4.7 ± 0.2 |
Physical Properties
Parameter | Details |
---|
Dehydrated Medium | Free-flowing, homogeneous, off-white |
Prepared Medium | Slightly opalescent, very light amber |
Environmental and Storage
Condition | Details |
---|
Storage Temperature | 10-30°C (Dehydrated Medium) |
| 2-8°C (Prepared Medium) |
Shelf Life | 4 years |
Packaging Options
Reference | Content | Packaging |
---|
610173 | 500 g | Plastic Bottle |
620173 | 100 g | Plastic Bottle |
Applications
- Food Safety:
- Ensures fungal safety in food and beverage products.
- Pharmaceutical Quality Control:
- Detects fungal growth in raw materials and finished products.
- Environmental Monitoring:
- Tracks mold and yeast growth in air and surfaces.
- Fungal Enumeration:
- Suitable for microbial counts in diverse industries.
Usage Guidelines
Preparation
- Suspend 31.3 g of powder in 1 liter of distilled or deionized water.
- Add 2.35 g of glycerol.
- Heat to boiling while stirring to ensure complete dissolution.
- Sterilize at 121°C for 15 minutes.
- Dispense into Petri dishes and allow to solidify.
Technique
- Inoculate the medium with 0.1 ml of diluted sample.
- Spread the inoculum with a sterile spatula.
- Incubate at 30 ± 2°C for 18-72 hours.
- Observe for fungal growth.
Interpretation of Results
- Yeasts and molds will form visible colonies on the agar surface.
Microbial Performance
Microorganism | ATCC® | Growth |
---|
Aspergillus niger | 16404 | Good |
Candida albicans | 10231 | Good |
Saccharomyces cerevisiae | 9763 | Good |
Advantages
- Enhanced Fungal Detection:
- Promotes consistent fungal growth while inhibiting bacteria.
- Easy Preparation:
- Streamlined process ensures reproducible results.
- Extended Shelf Life:
- Stable for up to 4 years when stored properly.
- Industry-Standard Compliance:
- Suitable for regulatory and industrial fungal testing.
Warnings and Precautions
- Use only by trained laboratory personnel.
- Store in a dry, dark environment to prevent contamination.
- Dispose of waste in accordance with local regulations.
References
- Reddish (1919). Abstr. Bacteriol. 3:6.
- Thom and Church (1926). The Aspergilli.
- AOAC Official Methods of Analysis (1995).
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