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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Solid |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
Medium for Cultivation of Yeasts and Moulds — USP/EP/JP Compliant
Liofilchem® Sabouraud Dextrose Broth (SDB) is a sterile, ready-to-use or dehydrated microbiological medium specifically designed for the cultivation and subculture of fungi, including yeasts and molds, from clinical and non-clinical materials. It is also widely used in the microbiological examination of non-sterile pharmaceutical, cosmetic, and personal care products, in accordance with harmonized pharmacopoeial standards (USP <62>, EP 2.6.13, JP 4.05).
This nutrient-rich medium provides the essential conditions for fungal recovery, including an acidic pH and high glucose concentration that support selective fungal growth while inhibiting most bacteria. It may also be supplemented with chloramphenicol for enhanced bacterial inhibition, especially in dermatophyte recovery.
Key Features and Benefits
- ✅ Pharmacopoeia-compliant (USP/EP/JP harmonized)
- ✅ Supports growth of dermatophytes, yeasts, and molds
- ✅ Liquid format ideal for direct inoculation or enrichment procedures
- ✅ Can be supplemented with antibiotics (e.g., chloramphenicol) for selective recovery
- ✅ Suitable for both clinical diagnostics and industrial microbiology applications
Typical Formula (per liter)
| Component | Quantity |
|---|---|
| Pancreatic Digest of Casein | 5.0 g |
| Peptic Digest of Animal Tissue | 5.0 g |
| Dextrose | 20.0 g |
| Final pH at 25 °C | 5.6 ± 0.2 |
Preparation Instructions (for dehydrated medium)
- Suspend 30 g of powder in 1 L of distilled or deionized water.
- Mix thoroughly and bring to boil with frequent agitation until fully dissolved.
- Dispense into appropriate containers.
- Sterilize by autoclaving at 121 °C for 15 minutes.
Test Procedure and Incubation
- Clinical use: Inoculate directly and incubate aerobically at 25 °C for 2–7 days.
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Industrial use:
- For Candida albicans: Incubate at 30–35 °C for 3–5 days.
- For Aspergillus brasiliensis: Grow at 20–25 °C for 5–7 days.
- Subculture on Sabouraud Dextrose Agar (SDA) if needed.
Interpretation of Results
- Positive result: Turbidity indicates fungal growth.
- Negative result: No turbidity; no fungal growth observed.
Quality Control (QC)
| Organism | ATCC® Code | Expected Growth |
|---|---|---|
| Candida albicans | ATCC® 10231 | Good |
| Aspergillus brasiliensis | ATCC® 16404 | Good |
| Saccharomyces cerevisiae | ATCC® 9763 | Good |
Incubation:
- C. albicans: 32.5 ± 2.5 °C for 48–72 hours
- Other organisms: 22.5 ± 2.5 °C for up to 5 days
- Atmosphere: Aerobic
Appearance
- Dehydrated medium: Free-flowing, homogeneous, light beige powder
- Prepared medium: Clear to slightly hazy light amber; slight precipitate may be present
Packaging Presentations
| Format | Content | Code |
|---|---|---|
| Tubes | 20 × 10 mL | 24109 |
| Bottles | 6 × 100 mL | 402040 |
| Bottles | 25 × 100 mL | 452040 |
| Bottles | 6 × 500 mL | 471070 |
| Dehydrated medium (powder) | 500 g | 610104 |
| Dehydrated medium (powder) | 100 g | 620104 |
Storage and Shelf Life
- Dehydrated medium: Store at 10–30 °C in a dry, tightly sealed container; shelf life: 4 years
- Prepared medium (tubes/bottles): Store at 10–25 °C protected from light; shelf life: 2 years
- Do not use if contaminated, expired, or deteriorated.
Liofilchem® Sabouraud Dextrose Broth is a trusted, pharmacopoeia-compliant solution for cultivating yeasts and molds in both clinical and industrial settings. Its robust formula and versatile formats make it ideal for applications ranging from fungal diagnostics to microbial quality control of non-sterile products. Produced under strict quality systems, it ensures reliable performance in mycological workflows.