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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | BD Biosciences | ||
| Product Grade | Molecular Biology | ||
High-sensitivity assay kit for detecting interferon-gamma secreting mouse cells by ELISPOT
The BD™ ELISPOT Mouse IFN-γ ELISPOT Set is a complete, research-use-only (RUO) kit designed for the highly sensitive and specific detection of mouse interferon-gamma (IFN-γ)–secreting cells using the enzyme-linked immunospot (ELISPOT) assay. This assay is ideal for analyzing immune responses at the single-cell level, even at very low frequencies (as rare as 1 in 300,000 cells).
Based on the principles of a sandwich ELISA, this ELISPOT assay employs high-affinity capture and detection antibodies specific for murine IFN-γ, along with enzyme amplification for visualizing discrete cytokine-producing cells. The kit is optimized for routine use in immunology research, including T cell monitoring, vaccine efficacy studies, and cytokine profiling.
Key Features
- High Sensitivity: Detects IFN-γ–producing cells at low frequencies (≤1/300,000)
- Quantitative: Enables enumeration of individual cytokine-secreting cells
- Specific & Reliable: High-affinity antibodies for murine IFN-γ ensure specificity
- Complete Kit: Contains pre-coated ELISPOT plates, capture/detection antibodies, and enzyme conjugate
- ELISPOT Reader Compatible: Suitable for use with modern ELISPOT analyzers for automated counting
- Validated Application: ELISPOT (Routinely Tested)
Applications
- Enumeration of antigen-specific T cells secreting IFN-γ
- Assessment of cellular immune responses in vaccine and infectious disease studies
- Monitoring of T cell activation in immuno-oncology
- Cytokine profiling in autoimmunity, transplantation, and allergy research
- Analysis of Th1-type responses in murine models
Kit Contents (for 10 Plates)
| Component | Description |
|---|---|
| ELISPOT Plates | 10 pre-coated, 96-well plates |
| Capture Antibody (Unlabeled) | For initial cytokine capture |
| Detection Antibody (Biotinylated) | For signal amplification |
| Enzyme Conjugate | For chromogenic detection |
| Certificate of Analysis | Batch-specific performance documentation |
Technical Specifications
| Specification | Details |
|---|---|
| Catalog Number | 551083 |
| Brand | BD™ ELISPOT |
| Application | ELISPOT (validated) |
| Analyte | Mouse IFN-γ (Interferon gamma) |
| Species Reactivity | Mouse |
| Assay Format | Sandwich ELISPOT |
| Plate Format | 96-well (10 plates included) |
| Regulatory Status | RUO (Research Use Only) |
| RRID | AB_2868922 |
The BD™ ELISPOT Mouse IFN-γ ELISPOT Set provides a highly sensitive and specific platform for detecting mouse cytokine responses at the single-cell level. With high-affinity antibodies and reliable components, this kit is ideal for immunological studies that require accurate quantification of IFN-γ–secreting cells, such as vaccine trials, infection models, and immuno-monitoring.