Applied Biosystems™ TaqMan™ QSY Probe with HPLC Purification

The Applied Biosystems™ TaqMan™ QSY Probe is a state-of-the-art dual-labeled probe designed specifically for high-performance real-time PCR (qPCR) applications. It is optimized for sensitivity and accuracy in multiplexed experiments, allowing detection of up to six targets simultaneously. Each probe undergoes rigorous HPLC purification and mass spectrometry verification to ensure lot-to-lot reproducibility and reliability in experimental results.

Key Features:

1. Enhanced Sensitivity:
   • Detects low-abundance targets, impurities, and fluctuations in bioproduction with greater accuracy than probes from other suppliers.
2. Seamless Multiplexing:
   • Compatible with up to four fluorophores (ABY, JUN, FAM, VIC) in standard multiplexing or up to six targets using advanced instrumentation.
   • Provides uncompromised signal clarity, enabling efficient simultaneous detection of multiple analytes.
3. Scalability:
   • Maintains consistent performance from singleplex to 6-plex assays, streamlining assay development and enabling smoother scalability.
4. Wide Dynamic Range:
   • Offers greater dynamic range than other leading suppliers, allowing precise quantification over a broader range of analyte concentrations.
5. BHQ Compatibility:
   • Existing Black Hole Quencher™ (BHQ™) probe designs can be converted to QSY without requiring sequence redesign, making the transition seamless for labs already using BHQ.
6. Instrument Compatibility:
   • Validated with Applied Biosystems™ instruments and master mixes, including QuantStudio™, StepOne™, and ViiA™ 7 systems, for assured compatibility and reliable results.
7. qPCR Workflow Integration:
   • Designed as part of Thermo Fisher Scientific's complete qPCR ecosystem, providing end-to-end support for assay development and execution.
8. Calibration Ready:
   • Compatible with calibration plates for ABY, JUN, and Cyanine 5, ensuring accuracy across 96-well (0.1 mL and 0.2 mL) and 384-well formats.

Specifications:

• 3′ Primer Modification: QSY quencher
• 5′ Primer Modification: ABY, FAM, JUN, VIC fluorophores
• Detection Method: Primer-probe hybridization
• Purification Method: High-Performance Liquid Chromatography (HPLC)
• Form: Available as liquid or dried-down formulation
• Dynamic Range: Ideal for low- to high-concentration analytes
• Reaction Speed: Fast qPCR
• Unit Size: 6000 pmol per tube
• Storage: Store at -15°C to -25°C

Applications:

1. Gene Expression Analysis:
   • Ideal for quantifying RNA/DNA levels in various experimental contexts.
2. Mutation and SNP Detection:
   • Detect specific mutations or single-nucleotide polymorphisms (SNPs) with high specificity.
3. Multiplex qPCR:
   • Efficiently analyze multiple targets in a single reaction.
4. Diagnostics and Bioproduction:
   • Monitor impurities and ensure process consistency in diagnostic or bioproduction workflows.
5. Pathogen Detection:
   • Rapid detection and quantification of bacterial, viral, or fungal targets in research applications.

Advantages over Other Probes:

• Performance Retention: Superior retention of sensitivity and accuracy even in highly multiplexed assays.
• Ease of Use: No redesign required for labs transitioning from BHQ probes.
• High Reliability: HPLC purification and mass spectrometry validation ensure consistent probe quality and performance.

Instrumentation Compatibility:

The TaqMan™ QSY Probe is compatible with a range of Applied Biosystems™ real-time PCR instruments, including:

• QuantStudio™ 3, 5, 6 Flex, 7 Flex, 12k Flex
• StepOne™ and StepOnePlus™ systems
• 7500 and 7900HT systems
• ViiA™ 7 System

Contents:

• 1 tube containing 6000 pmol of dual-labeled probe (available in liquid or dried-down form).

Storage and Shipping:

• Storage Conditions: Store at -15°C to -25°C.
• Shipping Conditions: Shipped at room temperature.

References:

1. Thermo Fisher Scientific. (2024). Applied Biosystems™ TaqMan™ QSY Probe Data Sheet.
2. Applied Biosystems™ QuantStudio™ Multiplexing Optimization Guide.
3. Thermo Fisher Scientific TaqMan Probe Technology Overview.

This product provides a reliable and efficient solution for sensitive, multiplexed qPCR workflows, making it ideal for both academic and industrial research applications.