Gibco™ Medium 199, Earle's Salts (CO2)

Gibco™ Medium 199 was developed for nutritional studies of chick embryo fibroblasts and has become widely used in virology, vaccine production, and in vitro cultivation of various primary cell types. This medium has broad applicability across species and is particularly suitable for non-transformed cells.

Applications:

• Nutritional studies and cultivation of:
  • Chick Embryo Fibroblasts
  • Mouse Pancreatic Epithelium
  • Rat Lens Tissues
• Virology and Vaccine Production: Ideal for supporting the growth of primary cell explants.
• Research Use Only: Not for diagnostic or therapeutic purposes.

Key Features:

1. Enhanced Composition:
   • Includes adenine, adenosine, hypoxanthine, thymine, and additional vitamins, providing unique nutritional benefits.
   • Contains Earle's salts for use in CO₂ incubators.
2. Defined Additives:
   • With: L-glutamine, phenol red, sodium pyruvate, and sodium bicarbonate.
   • Without: HEPES buffer.
3. Environment Compatibility:
   • Requires a 5-10% CO₂ environment due to the sodium bicarbonate buffer system.
4. Sterile and Sustainable:
   • Sterile-filtered and manufactured under cGMP compliance with ISO 13485 standards.
   • Packaged with sustainability in mind.

Specifications:

• Concentration: 1X
• Form: Liquid
• Osmolality: 290 - 310 mOsm/kg
• Shelf Life: 12 months from the date of manufacture.
• Culture Environment: CO₂.
• Sterility: Sterile-filtered.
• Storage: 2–8°C, protected from light.

Shipping and Storage:

• Storage Conditions: 2–8°C; protect from light.
• Shipping Conditions: Ambient temperature.
• Shelf Life: 12 months.

Product Details:

• Catalog Number: 31150022.
• Quantity: 500 mL.
• Product Line: Gibco™.

Benefits of Medium 199:

• Flexibility in Applications: Broad species compatibility for primary and non-transformed cells.
• Nutrient-Rich Formula: Unique inclusion of nucleotides and vitamins.
• Reliable Quality: Produced under stringent manufacturing standards.