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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Enzymes | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Molecular Biology, Cell Culture | ||
Irradiated Pancreatic Protease for Routine Cell Culture and Tissue Dissociation
Gibco™ Trypsin 1:250 is a highly purified, irradiated protease preparation derived from porcine pancreas, designed for routine cell culture passaging and primary tissue dissociation. Widely used in adherent cell culture workflows, this trypsin formulation is tested for safety and performance, ensuring low endotoxin levels, traceability, and reliable enzymatic activity. Supplied in powder format (100 g) for flexible reconstitution, it allows precise control over enzyme concentration and buffer composition.
Manufactured under dual-site cGMP conditions in FDA-registered ISO 13485–certified facilities, Gibco™ Trypsin 1:250 offers high consistency, supply continuity, and regulatory support documentation for research and manufacturing workflows.
Key Features & Benefits
✅ Irradiated and Highly Active – Consistent enzymatic activity for robust cell detachment and tissue dissociation
✅ Tested for Endotoxin, Mycoplasma, and Contaminants – Ensures sterility and safety in sensitive applications
✅ No EDTA or Phenol Red – Suitable for customizable buffer systems and downstream applications
✅ Documented Lot Traceability & Regulatory Support – Includes animal origin documentation, irradiation certificates, and viral inactivation summary
✅ Manufactured Under cGMP & ISO 13485 – Dual-site production (USA & UK) ensures continuity and compliance
✅ Long Shelf Life (24 Months) – Stable under refrigerated storage (2–8°C)
Applications
- Routine Passaging of Adherent Mammalian Cells
- Detachment of Cells from Culture Surfaces
- Dissociation of Primary Tissue for Cell Isolation
- Stem Cell and Cancer Cell Culture Maintenance
- Research and Manufacturing Reagent Formulation
Technical Specifications
| Attribute | Details |
|---|---|
| Product Name | Gibco™ Trypsin (1:250), Powder |
| Catalog Number | 27250018 |
| Quantity | 100 g |
| Format | Powder in securitainer-capped vial |
| Source | Porcine pancreas |
| Chelators | None (No EDTA) |
| Additives | No phenol red |
| Concentration | 250× |
| Cell Type Compatibility | Mammalian (Adherent) |
| Tested For | Endotoxin, mycoplasma, bacterial, fungal, viral presence |
| Endotoxin Level | Low |
| Manufacturing Standard | cGMP, ISO 13485-certified, FDA-registered facilities |
| Storage Conditions | 2–8°C |
| Shipping Conditions | Ambient |
| Shelf Life | 24 months from date of manufacture |
| Regulatory Use | For research or further manufacturing only |
Gibco™ Trypsin (1:250), Powder is a trusted reagent for high-performance, consistent cell detachment and tissue dissociation in both academic research and bioproduction environments. With stringent quality testing, global manufacturing redundancy, and regulatory-ready documentation, it supports safe, scalable, and reproducible workflows across all cell culture applications.