Gibco™ PBS Tablets

Pre-Measured Phosphate-Buffered Saline (PBS) for Cell Culture & Laboratory Applications

The Gibco™ PBS Tablets provide a convenient and consistent phosphate-buffered saline (PBS) formulation for a wide range of cell culture and biochemical applications. These tablets are designed for easy preparation, eliminating the need for weighing and pH adjustment while ensuring reliable and reproducible buffer conditions.

Each 5 g PBS tablet is formulated without calcium, magnesium, and phenol red, making it ideal for cell washing, reagent preparation, and tissue transportation. Simply dissolve one tablet in 500 mL of distilled water, and the solution will automatically adjust to pH 7.45, requiring no further modifications.

Key Features & Benefits

✅ Convenient & Pre-Measured

• Eliminates weighing errors, ensuring consistent buffer preparation.
• Dissolves quickly and completely in distilled water.

✅ Optimal for Cell Culture Applications

• Does not contain calcium or magnesium, preventing interference with cell dissociation reagents like trypsin.
• Suitable for cell washing, dilutions, and reagent preparation.

✅ Physiologically Balanced Buffer

• Maintains osmolarity and pH stability (pH 7.45) for biological compatibility.
• Ensures gentle handling of cells and tissues without toxicity.

✅ Sterile & Reliable

• Manufactured under strict quality control for biochemical and cell culture applications.
• Can be sterile-filtered after preparation if required.

Technical Specifications

Use Cases & Applications

1. Cell Culture Washing & Maintenance

Primary Use:

• Used for washing cells before trypsinization or passaging to remove excess serum and inhibitors.
• Provides a physiologically stable environment for cells before dissociation.

Practical Example:

A cell culture lab working with adherent HEK293 cells uses Gibco™ PBS Tablets to wash cells before trypsinization, ensuring efficient detachment and cell viability.

2. Cell Transport & Tissue Handling

Primary Use:

• Maintains osmotic balance and pH stability when transporting cells or tissues.
• Provides an isotonic environment to prevent cell lysis.

Practical Example:

A biotech company shipping primary cells uses Gibco™ PBS as a transport medium, ensuring cells remain viable and undamaged during shipment.

3. Dilution of Cells for Counting & Experiments

Primary Use:

• Used as a diluent for counting cells using hemocytometers or automated cell counters.
• Ensures consistent and reproducible cell dilutions in flow cytometry assays.

Practical Example:

A research lab conducting flow cytometry analysis dilutes spleen-derived immune cells in PBS before staining and analysis, ensuring accurate cell enumeration and viability assessment.

4. Reagent & Buffer Preparation

Primary Use:

• Used as a universal diluent for preparing antibody solutions, enzyme reactions, and staining reagents.
• Compatible with Western blotting, ELISA, and immunohistochemistry protocols.

Practical Example:

A biochemistry lab preparing ELISA assays uses PBS to dilute primary and secondary antibodies, ensuring consistent binding affinity and reduced background noise.

5. Sample Washing in Molecular Biology

Primary Use:

• Used for washing nucleic acid samples, protein precipitates, and immunoprecipitation beads.
• Maintains biochemical stability in sensitive assays.

Practical Example:

A genomics research lab performing RNA extractions uses PBS to rinse spin columns, ensuring clean RNA elution without contamination.

How to Prepare Gibco™ PBS Solution

1. Dissolve one 5 g Gibco™ PBS tablet in 500 mL of distilled water.

2. Stir or shake gently until the tablet is completely dissolved.

3. The solution is ready to use—no pH adjustment required.

4. If needed for sterile applications, filter sterilize using a 0.22 μm membrane filter.

5. Store at room temperature or at 4°C for long-term storage.

Ordering Information

Why Choose Gibco™ PBS Tablets?

🧪 Pre-Measured & Convenient – No need for weighing or pH adjustments.

🧫 Cell Culture Compatible – Calcium- and magnesium-free, ensuring optimal cell dissociation.

⚡ Reproducible & Consistent – Maintains osmotic balance and pH stability.

💡 Multipurpose Use – Suitable for cell washing, reagent preparation, and biochemical assays.

📈 Manufactured for Research Use – Ensures high-quality performance in cell culture workflows.

The Gibco™ PBS Tablets provide a high-quality, pre-measured solution for cell culture, reagent preparation, and molecular biology applications. Their ease of use, reliability, and compatibility with sensitive experiments make them a valuable buffer for research laboratories.