Roche Abts™ Solution, Pack Of 3X100Ml

The Roche ABTS™ Solution is a ready-to-use chromogenic substrate for peroxidase-driven indicator reactions in enzyme-linked immunosorbent assay (ELISA). It is formulated with 2,2′-azino-di-[3-ethylbenzthiazoline sulfonate (6)] and hydrogen peroxide in a glycine/citric acid buffer. This solution offers high sensitivity and stability, providing excellent visual results with a distinct bluish-green color.

Key Features:

1. Ready-to-Use Format:
   • Simplifies workflows by eliminating the need for preparation.
   • Pre-formulated in a glycine/citric acid buffer for consistent performance.
2. High Sensitivity and Stability:
   • Demonstrated superior performance compared to substrates like 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), and O-tolidine (OT).
   • Yields highly sensitive, specific, and reproducible results.
3. Distinct Color Development:
   • Produces a bluish-green color as the reaction product.
   • Ideal for visual and photometric evaluation.
4. Compatible with HRP:
   • Designed for assays using horseradish peroxidase (HRP) conjugates.
5. Optimized for Photometric Evaluation:
   • Reaction product absorbs at 405 nm.
   • Reference wavelength (≥490 nm) is automatically subtracted by plate readers for background correction.

Specifications:

Applications:

1. ELISA Assays:
   • High-sensitivity detection for HRP-conjugated antibodies or proteins.
2. Enzyme Immunoassays:
   • Suitable for applications requiring stable and specific colorimetric substrates.

Biochemical/Physiological Actions:

• Substrate Functionality:
  • ABTS™ serves as a chromogenic substrate for HRP, enabling the detection of enzyme activity in ELISA assays.
  • Delivers a sensitive and specific colorimetric response, enhancing assay reproducibility and clarity.

Advantages:

1. Ease of Use: Ready-to-use solution saves time and ensures consistency.
2. High Sensitivity: Outperforms other substrates in ELISA applications.
3. Distinct Color Development: Bluish-green color enhances visual detection.
4. Photometric Compatibility: Optimized for use with plate readers at 405 nm.

Handling and Storage:

• Store as per manufacturer recommendations to maintain reagent stability.
• Note: For life science research only. Not for diagnostic use.

Legal Information:

• ABTS™ is a trademark of Roche.