The Invitrogen™ BrdU Labeling Reagent is a thymidine analog used for labeling and detecting proliferating cells in vivo or in cultured cell systems. By incorporating into newly synthesized DNA during the S-phase of the cell cycle, BrdU allows for monitoring DNA replication and cell proliferation with high specificity and sensitivity.
Key Features
- Broad Application:
- Suitable for in vivo and in vitro BrdU incorporation studies.
- Compatible with cultured cells and tissue samples.
- High Sensitivity:
- Enables precise detection of DNA synthesis during the S-phase.
- Reliable Performance:
- Provides consistent and reproducible results across various sample types.
- Flexible Usage:
- Ideal for cell cycle analysis, proliferation assays, and DNA replication studies.
- Easy Storage:
- Stable at 2°C to 8°C, ensuring convenient storage and usability.
Specifications
| Feature | Details |
|---|
| Application | DNA labeling in vivo or in cultured cells |
| Label or Dye | BrdU |
| Form | Liquid |
| Quantity | 15 mL |
| Unit Size | 15 mL |
| Storage Conditions | 2°C to 8°C |
| Shipping Condition | Wet Ice |
| Target Application | Research Use Only |
Applications
- Cell Cycle Analysis:
- Track and measure cell proliferation through BrdU incorporation during DNA synthesis.
- Proliferation Assays:
- Quantify dividing cells in various biological samples.
- DNA Replication Studies:
- Monitor and analyze DNA synthesis in specific cell populations.
- In Vivo Labeling:
- Administer BrdU to live organisms for dynamic tracking of proliferative cells.
- Tissue Analysis:
- Detect actively dividing cells in tissue sections.
Workflow for BrdU Labeling
- Preparation:
- Dilute BrdU to the desired working concentration.
- Add to cultured cells or inject in vivo for systemic labeling.
- Incubation:
- Allow sufficient time for BrdU incorporation (dependent on cell cycle timing).
- Detection:
- Use anti-BrdU antibodies for immunodetection in conjunction with fluorescence microscopy, flow cytometry, or ELISA.
Advantages
| Feature | Benefit |
|---|
| High Sensitivity | Detects DNA replication with high precision. |
| Versatility | Usable in both in vivo and in vitro studies. |
| Reproducibility | Provides consistent labeling across experiments. |
| Ease of Storage | Stable at 2°C to 8°C for long-term use. |
| Wide Compatibility | Compatible with multiple detection techniques (e.g., microscopy, flow cytometry). |
Comparison with Competitor Products
| Feature | Invitrogen™ BrdU Labeling Reagent | Sigma-Aldrich BrdU | Abcam BrdU Labeling Reagent | BD Biosciences BrdU Reagent |
|---|
| Quantity | 15 mL | 5 mL, 10 mL | 10 mL | 5 mL |
| Form | Liquid | Liquid | Liquid | Liquid |
| Detection Compatibility | Immunodetection, flow cytometry, microscopy | Same | Same | Same |
| Usability | In vivo and in vitro applications | Primarily in vitro | Primarily in vitro | In vivo and in vitro |
| Storage | 2°C to 8°C | Room temperature | 2°C to 8°C | 2°C to 8°C |
| Ease of Use | High | Moderate | High | High |
Suggested Pairing
- Detection Kits:
- Invitrogen™ Anti-BrdU Antibodies for immunofluorescence and flow cytometry.
- Assay Platforms:
- Use with Invitrogen™ Attune™ NxT Flow Cytometer for high-throughput cell cycle analysis.
- Tissue Section Labeling:
- Combine with Invitrogen™ imaging reagents for precise BrdU detection in tissue sections.
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