Thermo Scientific™ Invitrogen™ Fluoromount-G™ Mounting Medium

Fluoromount-G Mounting Medium is a water-soluble, clear liquid medium specifically designed for mounting slides after immunofluorescent staining. It provides enhanced preservation of fluorescent signals while minimizing photobleaching, making it ideal for fluorescence microscopy.

Key Features:

1. Water-Soluble Medium:
   • Compatible with aqueous staining protocols and does not require dehydration steps.
   • Suitable for specimens where the final step involves aqueous reagents.
2. Antifade Properties:
   • Reduces fluorochrome quenching and photobleaching, preserving fluorescence intensity during microscopy.
3. Convenient Options:
   • Available with or without DAPI for nuclear counterstaining. The DAPI variant provides a bright blue nuclear counterstain, simplifying slide preparation.
4. Semi-Permanent Seal:
   • Provides a reliable seal for slide storage at 2–8°C, allowing for prolonged preservation of specimens.
5. Low Autofluorescence:
   • The medium does not fluoresce, ensuring clean and clear imaging of fluorescence signals.
6. Refractive Index:
   • Reported refractive index of ~1.4, making it suitable for specimens less than 10 μm thick.
   • For oil immersion objectives requiring a higher refractive index (1.52), Invitrogen offers Prolong Glass or SlowFade Glass mountants.
7. Ready-to-Use:
   • Supplied as a 1X stock solution containing sodium azide as a preservative.

Applications:

1. Immunofluorescent Staining:
   • Used in mounting slides for specimens stained with fluorescent dyes, enabling high-quality imaging.
2. Fluorescence Microscopy:
   • Ideal for observing delicate fluorescent signals, such as antibody-based staining or fluorescently labeled probes.
3. DAPI Counterstaining:
   • The DAPI variant provides nuclear staining, enhancing image contrast for applications such as cell cycle analysis, chromatin visualization, or tissue imaging.
4. Prolonged Specimen Storage:
   • Semi-permanent mounting ensures the stability of samples during long-term storage or repeated imaging sessions.

Usage Instructions:

1. Slide Preparation:
   • Complete staining with immunofluorescence or other fluorescent dyes in an aqueous solution.
   • Remove excess buffer and ensure the specimen is free of large liquid residues.
2. Application:
   • Place a drop of Fluoromount-G on the slide over the stained sample.
   • Cover the sample with a coverslip and gently press to spread the medium evenly.
3. Curing and Storage:
   • Allow the slide to dry and seal before imaging. Store slides at 2–8°C to maintain fluorescence stability.
4. Microscopy:
   • Observe specimens under a fluorescence microscope, ensuring optimal excitation and emission settings for the fluorochromes used.

Specifications:

Advantages:

1. Preservation: Prolongs fluorescent signals for reliable and reproducible imaging.
2. Flexibility: Compatible with various fluorochromes and staining methods.
3. Ease of Use: Simple mounting protocol reduces preparation time.
4. Cost-Effectiveness: A single bottle provides sufficient reagent for multiple slides.

Invitrogen™ Fluoromount-G™ Mounting Medium is a trusted solution for fluorescence microscopy applications. Its antifade properties, compatibility with aqueous staining, and availability with or without DAPI make it versatile and efficient for both research labs and clinical studies.