Invitrogen™ eBioscience™ Monensin Solution (1000X), 1 mL

The Invitrogen™ eBioscience™ Monensin Solution (1000X) is a potent intracellular protein transport inhibitor designed to block protein transport to the Golgi complex (GC), resulting in the accumulation of proteins in the endoplasmic reticulum (ER). This reagent is highly effective in enhancing the detection of intracellular cytokines during the last hours of in vitro activation of cells. Monensin is widely used in intracellular staining protocols, particularly for flow cytometry applications, and is compatible with both mouse and human cytokine detection.

Key Features:

1. Protein Transport Inhibition:
   • Monensin disrupts protein transport to the Golgi complex, leading to intracellular retention of proteins in the ER for enhanced detection.
2. Cytokine Detection Enhancement:
   • Improves detection of intracellular cytokines such as IL-2, IFN-γ, and other secreted factors, making it ideal for immunological studies.
3. Wide Applicability:
   • Effective for intracellular cytokine detection in both mouse and human cells.
   • Recommended for use in combination with other inhibitors, such as Brefeldin A, for optimal results in specific assay systems.
4. 1000X Concentrated Solution:
   • A highly concentrated liquid formulation, designed for convenience and cost-effectiveness.
5. Flow Cytometry Ready:
   • Compatible with multicolor flow cytometry protocols and intracellular staining workflows.

Specifications:

Applications:

1. Intracellular Staining:
   • Enhances detection of intracellular cytokines and other proteins by blocking their secretion during cell activation.
2. Flow Cytometry:
   • Ideal for intracellular cytokine staining and analysis using multicolor flow cytometry.
3. Immunological Research:
   • Used in studies involving immune activation, cytokine profiling, and intracellular signaling pathways.
4. Protein Transport Studies:
   • Enables detailed investigation of intracellular trafficking and Golgi complex functions.

Key Benefits:

1. Enhanced Sensitivity:
   • Improves intracellular cytokine retention for more accurate detection.
2. Reproducible Results:
   • Provides consistent performance across various experimental setups.
3. Ease of Use:
   • Supplied as a 1000X concentrated solution, simplifying preparation and reducing waste.
4. Compatibility:
   • Works effectively with both mouse and human cells, making it suitable for a wide range of research applications.

Usage Instructions:

1. Dilute Monensin to the desired working concentration in cell culture medium.
2. Add to cells during the last hours of in vitro activation to retain intracellular cytokines.
3. Pair with appropriate fixation and permeabilization buffers for intracellular staining protocols.

Storage and Handling:

• Storage Conditions: Store at 2–8°C to maintain stability.
• Avoid freezing the reagent to preserve activity.

For Research Use Only:

This product is intended for research purposes only and is not suitable for diagnostic or therapeutic applications.