Thermo Scientific™ Colchicine 97%

Colchicine is a potent alkaloid-derived secondary metabolite with well-documented antimitotic, anti-inflammatory, and cytotoxic properties. It exerts its primary biochemical effect by binding to tubulin, thereby inhibiting its polymerization and disrupting microtubule dynamics, making it a critical tool in cell cycle arrest, mitotic inhibition, and apoptosis studies.

This Thermo Scientific preparation of colchicine is supplied as a light yellow crystalline powder with ≥97% purity (determined by HPLC) and is suitable for biological, pharmacological, toxicological, and cellular research applications.

Available Pack Sizes

Chemical & Physical Specifications

Biochemical Actions & Mechanism

• Primary Action: Binds to tubulin, inhibiting its polymerization
• Disrupts: Microtubule assembly and dynamics
• Cellular Effects:
  • Inhibits mitosis
  • Induces apoptosis
  • Activates the JNK/SAPK pathway
  • Suppresses cytokine secretion, fibroblast proliferation, and vesicle motility
• Used to study:
  • Leukocyte migration and diapedesis
  • Lysosomal degranulation
  • Collagen transport

Applications in Research

Colchicine has been used in:

• Genotoxicity assays: Sister-chromatid exchange (SCE) studies
• Antiviral/antibacterial research: Cytotoxic activity against pathogens
• Cytoskeletal studies: Microtubule depolymerization models
• Calcium signaling studies: Investigating Ca²⁺ transients in taxol-treated cells
• Anti-inflammatory and immunological research: Models of pericarditis, gout, familial Mediterranean fever, and Behçet’s disease

Storage and Handling

• Powder Stability: Stable at room temperature
• Light Sensitivity: Colchicine darkens upon light exposure; store protected from light
• Solution Stability:
  • Autoclavable and filter-sterilizable
  • Stable ≥6 months in light-protected, sterilized solutions
  • Stable in neutral and slightly alkaline solutions (pH ~8.1) when frozen

Thermo Scientific™ Colchicine (≥97%) is a high-purity, well-characterized small molecule essential for microtubule-targeted research, mitosis inhibition, and anti-inflammatory studies. With broad utility in cell biology, oncology, toxicology, and drug development, colchicine remains a foundational compound for investigating cytoskeletal function, inflammation, and cell cycle regulation. Its solid form is stable at room temperature and is packaged securely for laboratory use.