Thermo Scientific™ ABTS (Diammonium 2,2'-azinobis[3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate]) ≥98%

ABTS is a water-soluble compound widely used as a chromogenic substrate for horseradish peroxidase (HRP) and other peroxidases in enzyme-linked immunosorbent assays (ELISA) and other biochemical applications. It is known for producing a green reaction product that can be measured spectrophotometrically.

Key Features:

1. Chemical Properties:
   • Formula: C₁₈H₂₄N₆O₆S₄
   • Molecular Weight: 548.68 g/mol
   • Purity: ≥98%
   • CAS Number: 30931-67-0
   • MDL Number: MFCD00010404
   • EINECS: 250-396-6
2. Application:
   • Used in colorimetric detection in ELISA assays and other peroxidase-based assays.
   • Reacts with HRP to produce a green chromogenic product measurable at 405 nm (primary absorbance peak).
3. Storage and Stability:
   • Store at ambient temperature to maintain stability.
   • Supplied in glass bottles for solid reagents.
4. Formats Available:
   • 1 g: Catalog Number J65535.03.
   • 5 g: Catalog Number J65535.06.

Applications:

1. ELISA and Enzyme Assays:
   • ABTS acts as a substrate for HRP and is used for sensitive detection of antigens or antibodies in immunoassays.
2. Kinetic and Endpoint Assays:
   • Suitable for both kinetic studies and endpoint assays in biochemical and molecular biology research.
3. Alternative Substrate for HRP:
   • Offers an alternative to substrates like TMB and OPD, particularly for assays requiring moderate sensitivity and stability.

Advantages:

Physical and Chemical Properties:

Available Pack Sizes:

Safety and Handling:

• Follow safety guidelines outlined in the Safety Data Sheet (SDS).
• Use proper personal protective equipment (PPE) while handling the compound.