Thermo Scientific™ Streptomycin sulphate

Streptomycin sulfate is a water-soluble aminoglycoside antibiotic derived from Streptomyces griseus. It is widely used in research and industrial applications due to its bactericidal activity against a broad spectrum of gram-negative and some gram-positive bacteria. Streptomycin inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, leading to misreading of mRNA and interruption of protein elongation.

Product Specifications:

• Chemical Formula: C₂₁H₃₉N₇O₁₂·1.5H₂SO₄
• Molecular Weight: 1457.4 g/mol
• Appearance: White to off-white powder
• Solubility: Soluble in water
• Loss on Drying: ≤7%
• Sulfate Content (SO₄): 18.0–21.5%

Storage and Stability:

• Storage Temperature: Refrigerator (2–8°C)
• Shelf Life: Ensure storage in a tightly sealed container to prevent moisture absorption. Protect from prolonged exposure to air and light.

Applications:

1. Microbiology:
   • Selective antibiotic for suppressing bacterial contamination in cell culture.
   • Used in selective media for isolating gram-negative bacteria.
2. Molecular Biology:
   • Prevents bacterial contamination in cell culture experiments.
   • Key component in growth media for recombinant protein expression.
3. Antimicrobial Research:
   • Studies on bacterial protein synthesis inhibition.
   • Research on antibiotic resistance mechanisms.
4. Veterinary Medicine:
   • Historical use for treating bacterial infections in livestock.
5. Pharmaceutical:
   • Ingredient in the production of pharmaceutical-grade antibiotics.

Mechanism of Action:

• Streptomycin binds to the bacterial 30S ribosomal subunit, disrupting the translation process.
• It causes misreading of the genetic code, leading to production of non-functional or toxic proteins.
• The antibiotic’s bactericidal effect occurs predominantly against actively dividing bacteria.

Key Features:

• Broad-spectrum activity against gram-negative and some gram-positive bacteria.
• High solubility in aqueous solutions for easy preparation.
• Ideal for use in cell culture applications to minimize contamination risks.

Preparation and Use:

1. Solution Preparation:
   • Dissolve in sterile water to prepare stock solutions.
   • Typical working concentration: 50–100 µg/mL in cell culture applications.
2. Compatibility:
   • Can be used in combination with other antibiotics, such as penicillin or kanamycin, for broader antibacterial coverage.

Safety and Handling:

• Hazard Warnings: Classified as an irritant; may cause respiratory, skin, and eye irritation.
• Precautions:
  • Handle with appropriate personal protective equipment (PPE).
  • Avoid inhalation, ingestion, and direct skin contact.
  • Dispose of in accordance with local regulations for antibiotic waste.

Chemical Identifiers:

• MDL Number: MFCD00037023
• CAS Number: 3810-74-0

Quality Assurance:

• Product quality is ensured through rigorous testing to meet industry standards for sulfate content and moisture levels, ensuring reproducibility in experimental and industrial applications.