Thermo Scientific™ Potassium-4-nitrophenyl sulphate 99+%

High-Purity Sulfatase Substrate for Enzymology and Biochemical Research

Potassium 4-nitrophenyl sulfate is a chromogenic arylsulfatase substrate, widely used in enzymology, biochemical assays, and cell-free systems to detect sulfatase activity through the release of a chromogenic product. The compound exhibits high purity (≥99%) and is ideal for sensitive applications requiring quantitative or qualitative assessment of arylsulfatase enzyme function.

This substrate yields 4-nitrophenol, a yellow-colored compound with strong absorbance near 405 nm, upon hydrolysis by arylsulfatases, enabling straightforward spectrophotometric detection.

Key Applications

• ✅ Sulfatase Activity Assays: Standard substrate in aryl sulfatase detection in biological samples, including tissues, fluids, and microbial cultures.
• ✅ Enzyme Inhibition Studies: Utilized in inhibitor screening assays targeting arylsulfatase activity.
• ✅ Model Compound: Serves as an inorganic analog of organic aryl sulfate esters in mechanistic enzymology studies.
• ✅ Glycosidase-Based Enzyme Assays: Used in combination with p-nitrophenyl glycosides to study exoglycosidase function.

Biochemical Properties

Features & Benefits

• High Sensitivity: Produces a measurable color change upon enzymatic cleavage, suitable for microplate and cuvette assays.
• Reproducible Results: High chemical purity (≥99%) ensures minimal background and reliable quantification.
• Versatile Application: Suitable for academic research, clinical diagnostics, and microbial enzyme studies.
• Research-Grade Quality: Trusted for routine use in arylsulfatase kinetics, mechanistic enzyme characterization, and chromogenic detection platforms.

Example Use Cases

1. Enzyme Assay in Marine Invertebrates: Substrate for measuring arylsulfatase activity in cell-free coelomic fluid of echinoderms.
2. Glycosidase-Enzyme Coupled Systems: Used in studies requiring substrate analogs to track p-nitrophenol release.
3. Enzymatic Inhibition Screening: Employed as a competitive inhibitor or reporter substrate in sulfatase-related studies.
4. Synthetic Analog: Mimics natural aryl sulfate esters for evaluating enzymatic reaction mechanisms.

Thermo Scientific™ Potassium 4-nitrophenyl sulfate (≥99%) is a reliable and sensitive chromogenic substrate for aryl sulfatase assays, combining high purity, water solubility, and optical detectability. It is a valuable reagent in enzymatic diagnostics, mechanistic biochemistry, and inhibitor profiling, offering consistent and high-quality results for researchers working with sulfatases or related enzymes.