Thermo Scientific™ Oxoid ™ Tryptone Soya Agar Ep/Usp/Jp/Bp (Dehydrated)

Thermo Scientific™ Oxoid™ Tryptone Soya Agar (TSA) is a versatile, nutrient-rich general-purpose medium formulated for the cultivation of a broad spectrum of microorganisms, including aerobic, anaerobic, and facultative bacteria. Also known as Casein Soya Bean Digest Agar, TSA conforms to the standards of major international pharmacopoeias including EP, USP, JP, and BP, making it suitable for pharmaceutical sterility and growth promotion testing, as well as clinical and food microbiology.

This dehydrated medium contains two high-quality peptones derived from pancreatic and enzymatic digestion, providing a rich source of amino acids and nitrogenous compounds. TSA supports robust microbial growth and is commonly used as a reference medium for evaluating selective media, as well as for preparing specialized media such as blood agar, chocolate agar, and enhanced hemolysis agar (EHA).

Key Features and Benefits

Typical Formula (per liter of medium)

Preparation Instructions

To prepare 1 L of medium:

1. Suspend 40 g of TSA powder in 1 L distilled water.
2. Heat to boiling with frequent stirring until fully dissolved.
3. Autoclave at 121°C for 15 minutes.

For Blood Agar:

• Cool to 45–50°C and add 7% sterile defibrinated blood. Mix gently.

For Chocolate Agar:

• Heat blood-supplemented TSA until it turns chocolate brown, indicating hemolysis.

Applications

• Pharmaceutical Microbiology
  • Sterility testing, growth promotion testing, and microbial enumeration.
• Food and Beverage Microbiology
  • Detection and enumeration of aerobic bacteria in food, dairy, and beverages.
• Clinical Diagnostics
  • Cultivation of clinically relevant organisms including Staphylococcus, Streptococcus, and Candida.
• Environmental Monitoring
  • Used in cleanroom and controlled environment sampling.
• Media Customization
  • Base for preparing enriched media like blood agar and chocolate agar.

Quality Control (Growth Promotion Testing)

Pharmaceutical QC (per pharmacopoeia guidelines)

Non-Pharmaceutical QC

Specifications

Ordering Information

Precautions

• Use aseptic technique to prevent contamination during preparation and handling.
• Hemolytic reactions on blood agar may vary depending on the species and source of blood used (e.g., sheep vs. horse).
• For laboratory use only. Not intended for therapeutic or diagnostic use in humans.

Thermo Scientific™ Oxoid™ Tryptone Soya Agar (TSA) is an industry-standard, pharmacopoeia-compliant medium for general microbiological cultivation and regulatory testing. With its broad compatibility and versatility, it serves as a reliable foundation for microbiological workflows in pharmaceutical, clinical, environmental, and food safety laboratories.