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Thermo Scientific™ Oxoid™ MRS Agar (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
Thermo Scientific™ Oxoid™ MRS Agar (Dehydrated) is a highly selective and nutritionally optimized medium developed by de Man, Rogosa, and Sharpe for the cultivation of lactic acid bacteria (LAB). It is the solidified version of MRS Broth and is specifically formulated to support the growth of a wide range of LAB, including the slow-growing and fastidious strains of Lactobacillus brevis and Lactobacillus fermenti. The formulation also supports Streptococcus, Pediococcus, and Leuconostoc species. MRS Agar is ideal for isolating and enumerating LAB in food microbiology, clinical diagnostics, and probiotic research.
This dehydrated medium conforms to international industrial microbiology standards such as AFNOR, IDF, and NMKL, making it a reliable choice for quality control and regulatory testing workflows.
Key Features and Benefits
| Feature | Benefit |
|---|---|
| Selective for LAB | Promotes growth of fastidious lactic acid bacteria while inhibiting contaminants |
| Optimized Formula | Contains peptone, yeast extract, glucose, and essential salts for vigorous growth |
| Pharmacopoeia-Aligned | Referenced in AFNOR, IDF, NMKL standards |
| Versatile Applications | Supports dairy, food, clinical, and probiotic testing |
| Supports Anaerobic Growth | Suitable for incubation under microaerophilic or CO₂-enriched conditions |
| Cost-Effective | High yield per 500 g—prepares approximately 8.1 liters of medium |
| Reproducible Results | Ensures consistent colony morphology and enumeration accuracy |
Typical Composition (per liter of medium)
| Component | Amount (g/L) |
|---|---|
| Peptone & Yeast Extract | Nutrient-rich base |
| Glucose | 20.0 |
| Sodium Acetate, Ammonium Citrate | pH buffer and LAB growth enhancer |
| Magnesium, Manganese salts | Trace elements for LAB metabolism |
| Dipotassium Phosphate | Buffering agent |
| Polysorbate 80 | Fatty acid emulsifier |
| Agar | 15.0 (solidifying agent) |
| Final pH (at 25 °C) | 6.2 ± 0.2 |
Preparation Instructions
- Suspend 62 g of MRS Agar powder in 1 liter of distilled water.
- Heat to boiling while stirring until fully dissolved.
- Sterilize at 121 °C for 15 minutes in an autoclave.
- Cool to 45–50 °C before pouring into sterile Petri dishes or tubes.
Applications
-
Food Microbiology
- LAB enumeration in yogurt, cheese, fermented vegetables, and beverages.
-
Clinical Microbiology
- Cultivation and identification of probiotic LAB strains in clinical samples.
-
R&D Laboratories
- Microbial ecology, probiotic strain development, and functional food analysis.
Incubation Guidelines
| Condition | Temperature | Time |
|---|---|---|
| Thermophilic LAB | 42 °C | 2 days |
| Mesophilic LAB | 35 °C | 2 days |
| Mesophilic-Psychrotrophic | 30 °C + 22 °C | 2 + 1 days |
| Psychrotrophic LAB | 25 °C | 3 days |
Recommended atmosphere: anaerobic or microaerophilic with 5% CO₂ for optimal growth.
Quality Control
| Organism | Expected Result |
|---|---|
| Lactobacillus gasseri ATCC® 19992 | Good growth; pale straw-colored colonies |
| Negative Control (uninoculated plate) | No growth |
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Dehydrated powder |
| Quantity | 500 g (CM0361B), 2.5 kg (CM0361R), 5 kg (CM0361T) |
| Yield | Approx. 8.1 L per 500 g |
| Storage Temperature | 10–30 °C |
| Shelf Life | 2 years from manufacturing (check label) |
| Target Organisms | Lactic acid bacteria (LAB) |
| Compliance | AFNOR, IDF, NMKL |
Thermo Scientific™ Oxoid™ MRS Agar is a highly trusted, nutritionally enriched medium for selective cultivation of lactic acid bacteria across food, clinical, and probiotic microbiology workflows. Its superior performance, reproducibility, and flexibility under various incubation conditions make it an essential component in quality control, research, and diagnostic microbiology laboratories.
- Pack Size: 500g 2.5kg Plastic bucket, 5kg