Thermo Scientific™ Ethidium bromide 95%, pure

Ethidium bromide (EtBr) is a potent fluorescent intercalating agent widely used for the visualization of nucleic acids in agarose gel electrophoresis and other molecular biology applications. Supplied at ≥94% purity (titration), this red-to-dark purple crystalline powder fluoresces strongly under UV light when bound to double-stranded DNA or RNA.

Due to its high affinity for nucleic acids, Ethidium bromide enables sensitive detection of even low quantities of DNA. It is used primarily in post-staining or precast gel loading buffers and remains a standard in molecular diagnostics despite the availability of non-mutagenic alternatives.

Key Features

• ≥94% purity (titration), molecular biology grade
• High-sensitivity DNA stain for agarose and polyacrylamide gel electrophoresis
• Strong red fluorescence under UV light upon intercalation with DNA or RNA
• Soluble in water (1% solution yields a clear red solution with pH ~4.5)
• Supplied as a stable, crystalline powder for long shelf life
• Provided with batch-specific Certificate of Analysis and Safety Data Sheet

Chemical & Physical Properties

Packaging Options

Applications

• Agarose and polyacrylamide gel electrophoresis for DNA/RNA visualization
• Fluorescent staining of nucleic acids in gel-based and solution assays
• Standard reference dye for molecular diagnostics and academic training
• Tool for evaluating DNA-binding interactions and conformational changes
• Post-electrophoresis gel staining or inclusion in gel casting solutions

Safety & Handling

• Highly toxic and mutagenic—handle with extreme caution
• Wear appropriate PPE: nitrile gloves, lab coat, UV face shield or goggles
• Handle under a fume hood and avoid UV exposure during staining
• Use designated waste disposal containers for contaminated materials
• Store tightly sealed at room temperature, away from light and moisture
• Refer to SDS (ACRO17096) for detailed safety and disposal information

Thermo Fisher Scientific’s Ethidium Bromide 95% Pure provides a high-sensitivity, research-grade nucleic acid stain trusted by scientists worldwide. Its robust fluorescence properties and consistent performance make it ideal for routine electrophoresis applications, nucleic acid detection, and molecular biology teaching labs, where visual clarity and sensitivity are critical.