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Thermo Scientific™ EDTA (ethylenediamine tetraacetic acid) cell culture reagent
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Cell Culture | Formula | C₁₀H₁₆N₂O₈ |
EDTA (Ethylenediaminetetraacetic acid) is a widely used chelating agent in cell culture, molecular biology, biochemistry, and analytical chemistry. It is known for its ability to bind divalent metal ions such as Ca²⁺, Mg²⁺, Fe²⁺, and Zn²⁺, thereby inhibiting metalloprotease activity, aiding in cell dissociation, and stabilizing enzymes and nucleic acids.
Thermo Fisher Scientific supplies EDTA in high-purity solid form, specifically suited for cell culture and biochemical applications, ensuring consistent performance and minimal trace contamination.
Key Features
- High-purity, reagent-grade EDTA for research and cell culture
- Strong chelation capacity for divalent cations
- Assists in enzyme stabilization, nucleic acid preservation, and cell detachment
- Ideal for buffer preparation and protein extraction workflows
- Supplied as a white crystalline solid
- Stable at ambient temperatures with long shelf life
- Packaged in glass bottles for secure, contamination-free storage
- Supplied with batch-specific Certificate of Analysis and SDS
Chemical & Physical Properties
| Property | Specification |
|---|---|
| CAS Number | 60-00-4 |
| Chemical Formula | C₁₀H₁₆N₂O₈ |
| Molecular Weight | 292.25 g/mol |
| Appearance | White crystalline solid |
| Melting Point | ~245 °C (decomposes) |
| Boiling Point | 614 °C (at 1013 hPa) |
| Flash Point | >100 °C |
| Density (20 °C) | 0.86 g/cm³ |
| Storage Temperature | Ambient |
| Hazard Classification | Irritant (GHS07) |
| EINECS | 200-449-4 |
| REACH Registration | 01-2119486399-18 |
| MDL Number | MFCD00003541 |
| Merck Index | 14,3517 |
Packaging Options
| Pack Size | Packaging Type |
|---|---|
| 500 g | Glass bottle for solids |
| 1 kg | Glass bottle for solids |
Applications
- Chelation of metal ions in buffers and media
- Cell detachment and dissociation in tissue culture
- Enzyme stabilization and inhibition of metal-dependent proteases
- Preservation of nucleic acids in molecular biology workflows
- Analytical reagent in titrations and complexometric assays
- Heavy metal removal in water analysis and sample prep
Safety & Handling
- Classified as irritant—avoid inhalation and contact with eyes or skin
- Use in a well-ventilated area or fume hood
- Store in tightly sealed containers away from moisture
- Refer to SDS (msds_ALFAJ62948) for full safety and disposal information
EDTA, Cell Culture Reagent Grade – Thermo Fisher Scientific provides a versatile, high-purity chelator essential for applications ranging from cell culture and protein purification to nucleic acid preservation and analytical chemistry. Its robust metal-binding capacity and reliable performance make it an indispensable tool across diverse research workflows.
- Pack Size: 1kg 500g