Specifications:
Application | Protein Electrophoresis & Western Blotting | ||
Storage Temperature | Ambient | ||
Product Type | Buffer | Forms | Liquid |
Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
Product Grade | Molecular Biology | Formula | C₃H₅NO |
High-Quality Ready-to-Use Monomer Solution for PAGE of Proteins and Nucleic Acids
Thermo Scientific™ BioAcryl-P is a 30% (w/v) premixed solution of acrylamide and bisacrylamide in a 29:1 ratio, optimized for the electrophoretic separation of proteins and nucleic acids. The 29:1 ratio delivers a gel matrix suitable for standard SDS-PAGE and DNA sequencing gels, providing excellent resolution and polymerization consistency.
This high-quality formulation simplifies gel preparation and is suitable for routine laboratory use in protein identification, molecular weight analysis, and nucleic acid sequencing workflows.
Key Features & Benefits
- Pre-Mixed 30% Solution – Saves time and minimizes variability in gel casting
- Optimized 29:1 Ratio – Ideal balance of acrylamide (monomer) and bisacrylamide (crosslinker) for resolving a wide range of protein and nucleic acid sizes
- Ready to Use – Eliminates weighing and solution preparation steps
- High Purity and Stability – For reliable polymerization and consistent gel quality
- Compatible with SDS-PAGE and Native PAGE – Supports a variety of electrophoretic applications
Applications
- Protein Separation by SDS-PAGE
- Nucleic Acid Electrophoresis (e.g., DNA sequencing)
- Molecular Weight Estimation
- 2D Electrophoresis Gels
- Analytical and Preparative PAGE Workflows
General Description
-
BioAcryl-P is a 30% aqueous solution containing acrylamide and bisacrylamide in a 29:1 ratio, where:
- Acrylamide serves as the primary gel-forming monomer
- Bisacrylamide functions as the crosslinker to establish the gel matrix
- Forms stable polyacrylamide structures at room temperature when used with appropriate catalysts (e.g., APS and TEMED)
Technical Specifications
Attribute | Specification |
---|---|
Product Name | BioAcryl-P 30% Solution (29:1) |
Product Type | Acrylamide/Bisacrylamide Solution |
Concentration | 30% (w/v) |
Acrylamide:Bisacrylamide Ratio | 29:1 |
Form | Liquid |
Use | Gel electrophoresis |
Recommended Applications | SDS-PAGE, Native PAGE, DNA sequencing gels |
Storage Conditions | Store at 4°C, protect from light |
Regulatory Use | For Research Use Only |
Thermo Scientific™ BioAcryl-P (30%, 29:1) is a convenient and reliable solution for preparing polyacrylamide gels used in protein and DNA electrophoresis workflows. With a precise 29:1 acrylamide:bisacrylamide ratio, it delivers excellent resolution and reproducibility, making it ideal for routine use in molecular biology, proteomics, and genomics laboratories.
- Pack Size: 500 mL 1000 mL