Human Total Prostate Specific Antigen (Psa) Elisa Kit, 96 Tests

The Human Prostate-Specific Antigen (PSA) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of PSA levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into prostate health, cancer diagnostics, and related conditions.

Purpose

• Quantification of PSA Levels:
  • Detect and measure PSA concentrations in human biological samples to study prostate health and cancer biomarkers.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to ensure accurate and reliable quantification of PSA. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to PSA.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where PSA in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to PSA is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing PSA.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • PSA concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of PSA with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of PSA concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• PSA standards for standard curve generation.
• HRP-conjugated PSA detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Prostate Health Research:
  • Study PSA as a biomarker for prostate function and health.
• Cancer Research:
  • Investigate PSA levels in prostate cancer diagnosis and progression.
• Clinical Diagnostics Research:
  • Assess PSA as a biomarker for benign prostatic hyperplasia (BPH) and other prostate-related conditions.
• Therapeutic Development:
  • Monitor PSA levels during treatment for prostate cancer or other prostate disorders.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of PSA.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated PSA antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate PSA concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Prostate-Specific Antigen (PSA) ELISA Kit is a critical tool for researchers studying prostate health, cancer biomarkers, and related conditions. Its precision, user-friendly design, and broad compatibility make it indispensable for clinical and experimental applications.