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Specifications:
| Application | Cell Separation | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Cell Density Medium | Forms | Liquid |
| Product Brand | Cytiva | ||
| Product Grade | Cell Culture | ||
Cytiva Percoll™ is a colloidal silica-based density gradient medium coated with polyvinylpyrrolidone (PVP), designed for the isolation and purification of cells, organelles, viruses, and subcellular particles.
Its unique composition allows formation of self-generated or pre-formed gradients in balanced salt solutions, making it one of the most versatile and widely used reagents for density gradient centrifugation in cell biology and biochemistry research.
Percoll™ is isotonic, non-toxic, and biologically inert, enabling gentle and efficient separations without altering cell morphology or function.
Key Features
- Versatile Density Gradient Medium: Suitable for separation of cells, organelles, and macromolecular complexes.
- Silica-PVP Composition: Colloidal silica particles (15–30 nm) coated with polyvinylpyrrolidone for biological compatibility.
- Adjustable Gradients: Can form continuous or step gradients by centrifugation or gradient mixers.
- Physiological Compatibility: Used with balanced salt solutions, 0.25 M sucrose, or physiological saline.
- Biologically Inert and Isotonic: Maintains osmotic balance and cell integrity during centrifugation.
- High Recovery and Viability: Enables efficient cell separation with minimal mechanical or osmotic stress.
- Colorless to Slightly Yellow: Clear colloidal suspension for easy gradient visualization.
- Certificate of Analysis (CoA) available upon request.
Specifications
| Property | Description |
|---|---|
| Composition | Colloidal silica coated with polyvinylpyrrolidone (PVP) |
| Form | Liquid suspension |
| Appearance | Colorless to slightly yellow |
| Density Range | Adjustable depending on dilution medium |
| Applications | Cell, organelle, virus, and particle separation |
| Compatibility | Balanced salt solution, physiological saline, 0.25 M sucrose |
| Sterility | Non-sterile |
| For Use With | Centrifugation-based gradient separations |
| Storage Conditions | Room temperature |
| Intended Use | Research Use Only (RUO) |
Applications
- Cell Separation: Isolation of lymphocytes, macrophages, neutrophils, and other blood or tissue cells.
- Organelle Fractionation: Separation of mitochondria, lysosomes, nuclei, and peroxisomes.
- Virology: Purification of viruses and subviral particles.
- Subcellular Analysis: Fractionation of cellular components for proteomic or biochemical assays.
- Density Gradient Studies: Formation of continuous or discontinuous gradients for macromolecular separations.
Advantages
- Gentle on delicate cell types
- Reproducible and consistent density gradients
- Compatible with a wide range of centrifuge rotors
- Suitable for both analytical and preparative separations
- Long shelf life and batch-to-batch consistency
The Cytiva Percoll™ medium is a silica-based, PVP-coated density gradient reagent ideal for biological particle separation via centrifugation.
Offering exceptional reproducibility, compatibility, and efficiency, it remains a gold standard for cell, organelle, and virus purification in research laboratories worldwide.
Trusted for its biocompatibility, isotonicity, and performance, Percoll™ continues to be a foundational reagent in cell biology, virology, and molecular biochemistry research.
- Pack Size: 250 mL 1 LIter