Nano-Glo® Luciferase Assay System

The Nano-Glo® Luciferase Assay System is a high-sensitivity luminescent reporter system optimized for detecting NanoLuc® luciferase activity. With its bright, long-lasting signal and simple "add-mix-measure" protocol, this assay supports applications ranging from bench-scale research to high-throughput screening (HTS).

Key Features:

1. High Sensitivity:
   • NanoLuc® luciferase is 100x more sensitive than firefly and Renilla luciferases, enabling detection in challenging applications.
2. Long-Lasting Signal:
   • Glow-type signal with a half-life of 120 minutes in standard tissue culture media, ideal for batch processing or continuous analysis.
3. Add-Mix-Measure Simplicity:
   • Single-reagent addition eliminates the need for separate lysis or reagent injection steps, streamlining workflows.
4. Broad Compatibility:
   • Can be used directly on cells expressing NanoLuc® luciferase or on the culture media when luciferase is secreted.
5. Optimized for High-Throughput Screening:
   • Extended signal stability and low background formulation ensure reproducibility and sensitivity in HTS environments.

Applications:

1. Reporter Gene Assays:
   • Tracks gene expression with unmatched sensitivity and dynamic range.
2. High-Throughput Screening (HTS):
   • Optimized for use in drug discovery and large-scale screening workflows.
3. Protein Interaction Studies:
   • Monitors real-time interactions using luminescent NanoLuc® technology.
4. Promoter and Pathway Analysis:
   • Quantifies promoter activity and intracellular signaling events.
5. Cell Viability and Cytotoxicity:
   • Used to measure cell health when coupled with appropriate reporters.

Specifications:

Advantages:

1. Enhanced Sensitivity:
   • NanoLuc® technology allows detection of extremely low-abundance targets.
2. Extended Bright Light Output:
   • Optimized for batch and continuous processing, reducing variability.
3. Ease of Use:
   • Simplified protocol saves time and reduces assay optimization steps.
4. Versatile Applications:
   • Suitable for a wide range of luminescent reporter-based assays in basic research and drug discovery.
5. Low Background:
   • Increases sensitivity by reducing noise from the assay system.

Kit Components:

• Nano-Glo® Luciferase Assay Substrate: Provides the light-emitting reaction component.
• Nano-Glo® Luciferase Assay Buffer: Includes an integral lysis buffer for use directly on cells or media.

Ordering Information:

Storage and Handling:

• Storage: Store reagents at -20°C to maintain stability.
• Working Solution: Prepare reagent by mixing substrate and buffer immediately before use. Protect from light.

Use Cases:

1. Gene Regulation Studies:
   • Tracks dynamic changes in gene expression with exceptional sensitivity.
2. Pathway Analysis:
   • Monitors intracellular signaling pathways using NanoLuc® reporter constructs.
3. HTS Environments:
   • Enables reliable and reproducible large-scale compound screening.
4. Real-Time Protein Studies:
   • Quantifies protein interactions and stability in live cells.
5. Cytotoxicity Assays:
   • Measures cellular health and viability using luminescence as a readout.

The Nano-Glo® Luciferase Assay System combines superior sensitivity, long signal duration, and ease of use, making it a premier choice for luminescent assays in research and high-throughput settings. Its robust performance and low background ensure reliable and reproducible results across a wide range of applications.

For more details on NanoLuc® Luciferase, visit the NanoLuc® Luciferase Technology page.

NanoLuc® luciferase is 100X more sensitive than firefly and Renilla luciferase assays.

Assay Advantages

• Advanced reporter system. Bright NanoLuc® reporter allows more sensitive detection in challenging applications.
• Extended bright light output is optimized for batch and continuous-process handling.
• Simplified assay optimization. Add-and-read simplicity makes it easy to scale assays. No need for separate lysis and reagent injection steps.
• Low background formulation offers increased sensitivity.

Signal decay kinetics for Nano-Glo® Luciferase Assay Reagent.