Millipore NutriSelect® Plus Middlebrook 7H10 Agar Base

Middlebrook 7H10 Agar Base is a selective, solid culture medium for the isolation, cultivation, and antimicrobial susceptibility testing of Mycobacterium tuberculosis and other Mycobacterium species. It is widely used in clinical diagnostics and research for early detection and sensitivity testing of acid-fast bacteria. This medium provides essential nutrients and growth factors, ensuring robust growth while partially inhibiting contaminating bacteria through the inclusion of malachite green.

Key Features

1. Optimized for Mycobacteria
   • Formulated with inorganic salts, amino acids, and growth supplements to support the growth of slow-growing Mycobacterium species.
2. Selective Inhibition
   • Malachite green suppresses the growth of non-mycobacterial contaminants while allowing Mycobacteria to thrive.
3. Enriched with Growth Factors
   • Compatible with Middlebrook OADC Growth Supplement (M0678) to provide essential oleic acid, albumin, sodium chloride, dextrose, and catalase.
   • Albumin neutralizes toxic free fatty acids, while dextrose serves as an energy source.
4. Stable Solid Medium
   • Ideal for primary isolation, culture maintenance, and antimicrobial susceptibility testing.
5. Easy to Prepare
   • Powdered form ensures ease of preparation and consistent performance.

Specifications

Composition (per liter)

Preparation Instructions

1. Suspend 19.47 g of Middlebrook 7H10 Agar Base in 900 mL of distilled water.
2. Add 5 mL of glycerol (Cat. No. 49767).
3. Boil the mixture to dissolve the medium completely.
4. Distribute the medium in 180 mL amounts in Erlenmeyer flasks.
5. Autoclave at 121°C for 10 minutes under 15 lbs. pressure.
6. Cool the medium to 45°C.
7. Aseptically add 20 mL of Middlebrook OADC Growth Supplement (Cat. No. M0678).
8. Mix well and pour into sterile screw-capped tubes or Petri plates.
9. Keep the prepared medium protected from light before and after inoculation.

Applications

1. Isolation and Cultivation of Mycobacteria
   • Selective medium for Mycobacterium tuberculosis and other slow-growing Mycobacteria.
2. Antimicrobial Susceptibility Testing
   • Used in drug sensitivity testing to evaluate resistance to antibiotics, including rifampicin, isoniazid, and streptomycin.
3. Clinical Diagnostics
   • Primary culture for the detection and identification of acid-fast bacteria in clinical specimens (e.g., sputum, urine, biopsies).
4. Research Studies
   • Supports the growth of Mycobacterium bovis, M. avium, and M. smegmatis for vaccine development, drug resistance studies, and metabolic research.
5. Veterinary and Food Microbiology
   • Screening for Mycobacteria in animal specimens and food products.

Advantages

1. Selective for Mycobacteria
   • Malachite green inhibits non-mycobacterial contaminants while supporting robust Mycobacterial growth.
2. Improved Drug Susceptibility Testing
   • Stable medium ensures reliable results in antimicrobial testing protocols.
3. Rich Nutrient Composition
   • Contains essential growth factors and compatible with OADC Supplement for enhanced metabolism.
4. Solid Medium Stability
   • Solid agar ensures consistent colony formation and quantification.
5. Versatile Applications
   • Applicable to clinical, food, veterinary, and research microbiology.

Quality Control

Comparison with Middlebrook 7H9 and 7H11

The Middlebrook 7H10 Agar Base is a reliable, selective, and nutrient-rich medium tailored for the isolation, cultivation, and sensitivity testing of Mycobacterium tuberculosis and related species, making it indispensable for clinical, veterinary, and research laboratories. Let me know if you need further comparisons or additional insights!