Liofilchem Paromomycin PAR, 0.064-1024, MIC Test

The Liofilchem™ MTS™ Paromomycin (PAR) 0.064–1024 μg/mL MIC Test Strips are designed for precise determination of the Minimum Inhibitory Concentration (MIC) of paromomycin against various microorganisms. These strips are essential in assessing microbial susceptibility, guiding effective antimicrobial therapy, and monitoring resistance patterns.

Test Procedure:

1. Inoculum Preparation:
   • Select well-isolated colonies from an overnight culture.
   • Suspend the colonies in sterile saline or broth to achieve a turbidity equivalent to a 0.5 McFarland standard.
2. Inoculation of Agar Plate:
   • Dip a sterile swab into the prepared inoculum suspension.
   • Remove excess liquid by pressing the swab against the tube wall.
   • Evenly streak the swab over the entire surface of a Mueller-Hinton agar plate to ensure a uniform lawn of growth. 
3. Application of MTS™ Strip:
   • Allow the inoculated plate to dry for approximately 15 minutes at room temperature.
   • Using sterile forceps, place the MTS™ Paromomycin strip onto the agar surface with the concentration scale facing upward.
   • Ensure complete contact between the strip and the agar surface; do not reposition once applied. Liofilchem
4. Incubation:
   • Incubate the plate in an inverted position at 35 ± 2°C for 16–20 hours under ambient atmospheric conditions. 
5. Reading and Interpretation:
   • After incubation, observe the plate for an elliptical zone of inhibition around the strip.
   • Determine the MIC by identifying the point on the scale where the edge of the inhibition ellipse intersects the strip.
   • Interpret the MIC value using established clinical breakpoints from CLSI or EUCAST guidelines. Liofilchem

Quality Control:

To ensure the accuracy and reliability of the test, perform quality control using standard reference strains with known MIC values. For paromomycin, the following quality control strain is recommended:

Regular testing of this control strain ensures that the test conditions are optimal and that the MTS™ strips are performing within specified parameters. If the MIC values obtained for the control strain fall outside the expected range, investigate potential issues with the testing procedure, reagents, or equipment.

References:

• Liofilchem® MIC Test Strip Application Guide: https://www.liofilchem.net/pdf/mic/MIC_APPLICATION_GUIDE.pdf
• EUCAST Clinical Breakpoints: https://www.eucast.org/clinical_breakpoints

For detailed procedures, quality control ranges, and interpretative criteria, always refer to the manufacturer's instructions and the latest CLSI and EUCAST guidelines.