Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) mono, 60 Tests

The Thermo Scientific™ O.B.I.S. Mono Biochemical Identification System is a rapid, colorimetric test designed to differentiate Listeria monocytogenes from other Listeria species based on the detection of D-alanyl aminopeptidase (DALAase) activity. This innovative system provides fast and accurate results, simplifying the identification process for laboratories conducting microbiological testing.

Key Features

1. Rapid Testing:
   • Provides results in just 6 minutes, enabling quick decision-making.
2. Simple and Convenient:
   • Easy to implement in standard microbiology workflows, reducing complexity.
3. Specific Identification:
   • Reliably differentiates Listeria monocytogenes (lacking DALAase) from other Listeria spp.
4. Safe and Non-Carcinogenic:
   • Uses a non-carcinogenic substrate, addressing health concerns associated with older aminopeptidase tests.
5. Flexible Application:
   • Designed for the secondary biochemical testing of presumptive Listeria spp. isolated on selective media.

Specifications

Applications

1. Clinical Microbiology:
   • Rapid differentiation of Listeria monocytogenes in clinical samples.
2. Food Safety Testing:
   • Used in food laboratories to identify Listeria monocytogenes in foodborne pathogen testing workflows.
3. Environmental Monitoring:
   • Ideal for detecting Listeria monocytogenes in environmental samples from food production and processing facilities.

Advantages

• Efficiency: Reduces the need for a full biochemical identification of suspect colonies.
• Speed: Provides fast and reliable results in under 10 minutes.
• Safety: Incorporates modern substrates that are safer for laboratory personnel.
• Reliability: Differentiates based on specific biochemical activity, ensuring accuracy.

Testing Workflow

1. Isolate Colonies:
   • Begin with Listeria spp. presumptively identified on selective media.
2. Apply Test:
   • Use the O.B.I.S. Mono system to test for the presence of D-alanyl aminopeptidase (DALAase) activity.
3. Interpret Results:
   • Positive for DALAase: Non-Listeria monocytogenes species.
   • Negative for DALAase: Presumptive Listeria monocytogenes.
4. Confirm Results:
   • Use complementary tests as needed for further verification.

References

• Kampfer et al. (1991) - Physiological characterization and identification of Listeria species.
• Clark & McLauchlin (1997) - Colorimetric tests for differentiating Listeria monocytogenes.
• Bascomb & Manafi (1998) - Enzyme tests for Gram-positive cocci identification.

Packaging

• Quantity: 60 Tests/Kit.
• Catalog Number: ID0600M.

Why Choose O.B.I.S. Mono?

• Simplifies workflows for laboratories identifying Listeria monocytogenes.
• Provides accurate and rapid results, enhancing laboratory efficiency.
• Ensures safety with non-carcinogenic substrates.