GoTaq® G2 Flexi DNA Polymerase

The GoTaq® G2 Flexi DNA Polymerase is a versatile and high-performance DNA polymerase designed for PCR applications. It is a full-length Taq polymerase that provides reliable and consistent amplification of a wide range of DNA templates.

This enzyme features 5′→3′ exonuclease activity and is supplied in a Mg-free buffer system, allowing researchers to optimize Mg²⁺ concentrations for improved reaction conditions. The proprietary buffer system enhances amplification efficiency, gel loading convenience, and direct visualization of PCR products.

Key Features & Benefits

1. High Performance & Versatility

✔ Full-Length Taq DNA Polymerase – Ensures efficient and accurate DNA amplification.

✔ 5′→3′ Exonuclease Activity – Supports high-fidelity amplification without unwanted side reactions.

✔ Reliable Across a Wide Range of PCR Templates – Works with genomic, plasmid, and viral DNA.

2. Magnesium-Free Buffer System for Custom Optimization

✔ Mg-Free 5X Green & Colorless Buffers – Allows users to titrate Mg²⁺ for optimized PCR conditions.

✔ Green Buffer with Tracking Dyes – Blue and yellow dyes separate during electrophoresis, simplifying gel tracking.

✔ Colorless Buffer for Fluorescent or Absorbance-Based Applications – Ensures direct DNA quantification without interference.

3. Direct-to-Gel Convenience

✔ No Need for Loading Dye – The Green Buffer contains a density-enhancing compound that allows direct sample loading onto agarose gels.

✔ Efficient DNA Migration Tracking – The dyes provide a visual indicator of electrophoresis progress.

4. Enhanced Manufacturing for Reliability & Consistency

✔ High Purity & Stability – Manufactured under strict quality control processes to ensure batch-to-batch consistency.

✔ Reproducible PCR Results – Designed for high-yield and specific amplification of DNA fragments.

Technical Specifications

Applications

🔹 General PCR Applications – Routine DNA amplification for research, genotyping, and cloning.

🔹 High-Sensitivity PCR – Suitable for low-copy number templates and degraded DNA samples.

🔹 Genotyping & SNP Analysis – Amplifies DNA for single nucleotide polymorphism (SNP) detection.

🔹 cDNA & RNA-Related PCR – Used in RT-PCR workflows when paired with reverse transcription enzymes.

🔹 Mutation Analysis – Detects mutations and sequence variations in genetic research.

🔹 Microbial & Viral DNA Amplification – Works with bacterial, viral, and plasmid DNA templates.

🔹 DNA Library Preparation – Used in next-generation sequencing (NGS) and cloning workflows.

Kit Contents & Ordering Information

📦 Shipping Conditions: Dry Ice

🧊 Storage: -20°C for long-term stability

Why Choose GoTaq® G2 Flexi DNA Polymerase?

✅ Flexible Mg²⁺ Optimization – Allows researchers to adjust Mg²⁺ levels for different PCR requirements.

✅ Dual Buffer System for Different Applications – Green Buffer for direct gel loading, Colorless Buffer for fluorescence-based detection.

✅ Consistent & Reliable Performance – Ensures high-fidelity and robust PCR amplification.

✅ Compatible with Various PCR Workflows – Ideal for standard PCR, genotyping, and high-sensitivity applications.

The GoTaq® G2 Flexi DNA Polymerase is a powerful and flexible enzyme for PCR amplification, providing high efficiency, enhanced reliability, and customizable Mg²⁺ optimization. Its direct-to-gel Green Buffer and high-performance amplification capabilities make it ideal for routine PCR, genotyping, and research applications.

🚀 Ideal for: Molecular biologists, geneticists, microbiologists, forensic scientists, and clinical researchers conducting high-quality PCR assays.